Garlic protein zymolysis serial product and its preparation method and use
A technology of proteolysis and garlic, applied in the field of development and utilization of vegetable protein, can solve the problems such as deep processing of residues, not involving garlic protein, waste, etc.
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Embodiment 1
[0017] Embodiment 1, single enzyme hydrolysis (1)
[0018] Grind garlic dry residue after taking garlic oil, allicin or alliin, weigh 100g garlic dry residue powder, add distilled water to dissolve, add flavor protease according to the ratio of 5AU / kg, adjust pH to 6. Heat it in a constant temperature water bath to the enzyme action temperature of 40°C for 3 hours for enzymolysis. During the enzymatic hydrolysis process, the pH was constantly adjusted to keep its value at a constant value. After the end of the enzymolysis, heat and boil at 100° C. for 15 minutes to terminate the enzymolysis, and detect about 78% of the ACE inhibitory activity of the enzymolyzate.
Embodiment 2
[0019] Embodiment 2, single enzyme hydrolysis (2)
[0020] Weigh 100g of garlic dry residue powder, add distilled water to dissolve, add neutral protease at a ratio of 15AU / kg, adjust the pH to 7 with sodium hydroxide and hydrochloric acid, and keep the temperature at 30°C for 2 hours for enzymatic hydrolysis. During the enzymatic hydrolysis process, the pH was constantly adjusted to keep its value at a constant value. After the end of the enzymolysis, heat and boil for 15 minutes to terminate the enzymolysis, and detect about 82% of the ACE inhibitory activity of the enzymolyzate.
Embodiment 3
[0021] Embodiment 3, double enzyme hydrolysis
[0022] Weigh 100g of garlic dry residue powder, add distilled water to dissolve, add complex protease Protamex according to the ratio of 8AU / kg, adjust the pH to 8 with sodium hydroxide and hydrochloric acid, and keep the temperature at 50°C for 3 hours for enzymatic hydrolysis. The enzyme is then heated to inactivate it. Further add alkaline protease at a ratio of 10 AU / kg, adjust the pH to 9, and keep the temperature at 60° C. for 2 hours for enzymatic hydrolysis. After the enzymatic hydrolysis, the pH was adjusted to 7, the enzyme was inactivated, and the ACE inhibitory activity of the hydrolyzate was detected to be about 85%.
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