Bone marrow mononuclear cell infected by recombinant co-gene adeno-associated virus and use thereof

A nuclear cell, single technology, applied in the direction of cells modified by introducing foreign genetic material, recombinant DNA technology, medical preparations containing active ingredients, etc. death, avoidance of graft-versus-host reaction, and prevention of tissue edema

Inactive Publication Date: 2005-03-16
谭最
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Retroviruses can achieve efficient gene transfer and gene integration, but cannot be introduced into non-dividing cells and are pathogenic [15]
The adenovirus system can mediate the efficient transfer of genes in dividing and non-dividing cells, but cannot integrate into the host cell genome, and can only achieve transient transduction and expression of genes

Method used

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  • Bone marrow mononuclear cell infected by recombinant co-gene adeno-associated virus and use thereof
  • Bone marrow mononuclear cell infected by recombinant co-gene adeno-associated virus and use thereof
  • Bone marrow mononuclear cell infected by recombinant co-gene adeno-associated virus and use thereof

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Experimental program
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Embodiment Construction

[0029] The bone marrow mononuclear cells provided by the present invention are bone marrow mononuclear cells infected by recombinant common gene adeno-associated virus, namely AAVAngiopoietin1-VEGF, AAV Angiopoietin1 and AAV VEGF, which are used for the treatment of coronary heart disease, arteriosclerosis obliterans, diabetic arterial occlusion ischemic diseases such as syndrome, thromboangiitis obliterans, and its preparation method comprises the following steps:

[0030] (1) The early gene that affects human angiogenesis, that is, VEGF, and the late gene, that is, Angiopoietin1, are individually and combined, and the three are combined to form a cogene, which is constructed in the AAV plasmid system to form pAAV Angiopoietin1-VEGF, pAAV Angiopoietin1 and pAAV VEGF plasmids, That is, the pAAV plasmid system, wherein VEGF and Angiopoietin1 in the first plasmid each contain a set of CMV promoter and PolyA terminator system;

[0031] (2) The pAAV plasmid system was recombined i...

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Abstract

The invention relates to marrow monocyte infected with recombinant cogene AAV and its uses. Marrow monocyte infected with AAV Angiopoietinl-VEGFíóAAV Angiopoietinl and AAV VEGF is used in treating ischemia disease. The preparation steps include: Forming pAAV plasmid system containing single or united gene of blood vessel regeneration early gene and late gene, each plsmid containing a set of CMV promotor and Poly A teminator, recombining pAAV plamid system in AAV, forming three kinds of recombinant cogenen AAV, infecting blood vessel endothelial cell differentiated from marrow monocyte with AAV, injecting infected blood vessel endothelial cell ischemia tissue. The invention is safe and effective, has function of promoting blood vessel genesis.

Description

technical field [0001] The invention relates to the field of molecular cell biology, in particular to bone marrow mononuclear cells infected by recombinant cogenetic adeno-associated virus and its application. Background technique [0002] Cardiovascular ischemic disease is one of the persistent diseases that threaten human life and health. It mainly includes arteriosclerosis occlusive disease, diabetic arterial occlusive disease, and thromboangiitis obliterans. The above population is 10%, 37-69 years old is 1-2%, and the overall incidence rate in my country is 0.74%, and with the change of people's diet structure and the improvement of medical diagnosis level, the incidence rate has a rising trend. , has become a common and frequently-occurring disease in vascular surgery. The onset of this type of disease is hidden, the harm is serious, and multiple viscera and organs are often invaded, which can be fatal or disabled. However, although there are many methods for its treat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/15C12N5/10C12N15/66C12N15/85
Inventor 谭最陈德杰艾鹏
Owner 谭最
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