Nucleoprotamine and sodium alginate microcapsule and its preparation method

A technology of protamine and sodium alginate is applied in the directions of microcapsule preparation, microcapsule, microsphere preparation, etc., and achieves the effects of mild preparation conditions, low price, easy application and promotion

Inactive Publication Date: 2005-03-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

US2004 / 0013721A1 discloses a method for preparing polyelectrolyte sodium polystyrene sulfonate / polyallylamine hydrochloride microcapsules using LbL technology, which has biocompatibility problems in biomedical applications

Method used

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  • Nucleoprotamine and sodium alginate microcapsule and its preparation method
  • Nucleoprotamine and sodium alginate microcapsule and its preparation method
  • Nucleoprotamine and sodium alginate microcapsule and its preparation method

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Effect test

Embodiment 1

[0029] (1) First heat 1.36 g / L manganese sulfate solution and 1.8 g / L ammonium bicarbonate solution to 50°C respectively, then add sodium polystyrene sulfonate into the manganese sulfate solution, stir evenly, and finally stir under high speed , quickly add an equal volume of ammonium bicarbonate solution to the manganese sulfate solution, the concentration of sodium polystyrene sulfonate is 0.4 g / L, and react at a constant temperature of 50° C. for 1 hour to obtain manganese carbonate colloidal particles. The manganese carbonate colloidal particles are centrifuged and washed for later use. Gained colloidal particles are uniform in size and regular in appearance, and the particle size is about 1.5 microns (see figure 1 ).

[0030] (2) Add manganese carbonate colloidal particle dispersion to 2 g / L protamine sulfate (containing 29 g / L sodium chloride) aqueous solution, sonicate for one minute, then place it on an oscillator and vibrate for 20 minutes, until it is fully absorbed...

Embodiment 2

[0033] (1) Add sodium polystyrene sulfonate to 10 g / L of manganese sulfate solution, stir evenly, the concentration of sodium polystyrene sulfonate is 1 g / L, then under high-speed stirring, an equal volume of 8 g / L One liter of ammonium bicarbonate solution was quickly added to the manganese sulfate solution, and reacted at a constant temperature of 20° C. for 0.5 hour to obtain manganese carbonate colloidal particles. The manganese carbonate colloidal particles are centrifuged and washed for later use. The size of the obtained colloidal particles is about 10 microns.

[0034](2) Add manganese carbonate colloidal particle dispersion to 0.5 g / L protamine (containing potassium chloride 50 g / L) aqueous solution, sonicate for one minute, then place on an oscillator and vibrate for 10 minutes, until it is fully absorbed. Then through three times of "centrifugation / removal of supernatant / dispersion with high-purity water" to remove unadsorbed protamine. Then add 0.5 g / L sodium alg...

Embodiment 3

[0037] (1) Heat 0.1 g / L manganese sulfate solution and 0.1 g / L ammonium bicarbonate solution to 80°C respectively, under high-speed stirring, quickly add an equal volume of ammonium bicarbonate solution into the manganese sulfate solution, 80°C React at constant temperature for 2 hours to obtain manganese carbonate colloidal particles. The manganese carbonate colloidal particles are centrifuged and washed for later use. The size of the obtained colloidal particles is about 0.5 micron.

[0038] (2) Add manganese carbonate colloidal particle dispersion to 5 g / L protamine sulfate (containing 10 g / L sodium bromide) aqueous solution, sonicate for one minute, then place on an oscillator and vibrate for 60 minutes, until it is fully absorbed . Then through three times of "centrifugation / removal of supernatant / dispersion with high-purity water" to remove unadsorbed protamine. Then add 5 g / L sodium alginate (molecular weight of sodium alginate is 3.5 million, containing potassium ch...

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Abstract

A protamine-sodium alginate microsoftgel with shell-core structure is prepared through reaction between manganese sulfate, ammonium dicarbonate, and polystyrene-sodium sulfonate solution to obtain the colloid particles of manganese carbonate, dispersing in liquid, adding it to the aqueous solution of protamine, stirring, removing excessive protamine, adding the aqueous solution of sodium alginate, stirring, removing excessive sodium alginate, altermative adsorption to obtain shell-core structure and removing manganese carbonate by acid solution.

Description

technical field [0001] The invention relates to a microcapsule technology, in particular to a protamine and sodium alginate microcapsule and a preparation method thereof. Background technique [0002] In the early 1990s, Decher et al. (Thin Solid Films, 1992, 210 / 211: 832-835) first used layer-by-layer self-assembly technology (layer-by-layer, LbL) to assemble polyelectrolytes with opposite charges on a flat plate Nano-multilayer film, a technology that can precisely control the structure, composition, shape and thickness of the film at the nanoscale, has been rapidly applied to the preparation of microcapsules (Advanced Materials, 2001, 13: 11-22). Firstly, the polyelectrolyte with opposite charge is alternately adsorbed on the spherical template layer by layer, and then the template is removed to obtain polyelectrolyte microcapsules. This new type of microcapsule has potential applications in the fields of drug controlled release, chemical sensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K9/62B01J13/10
Inventor 王朝阳童真何成毅梁振鹏
Owner SOUTH CHINA UNIV OF TECH
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