Thyroid disease auxiliary diagnosis instrument and its application
A technology for thyroid diseases and diagnostic instruments, applied in the field of auxiliary diagnostic instruments for thyroid diseases, can solve the problems of high cost, time-consuming and labor-intensive, unsuitable for large-scale analysis, etc., and achieves the effects of simple method, simplified extraction process, and avoidance of sample preprocessing steps.
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[0025] 1. The instrument of the present invention can realize automatic pretreatment of blood samples, automatic sampling and automatic separation and quantification of coenzyme Q10 components; it is mainly completed through the following steps;
[0026] 1) Take a fresh plasma sample with a volume of 0.3ml, add 1ml of n-propanol (the volume ratio of the two can be 0.2:1 to 0.4:1) and centrifuge to degrease the protein, then directly load the supernatant for analysis;
[0027] 2) When the six-way valve is in the "LOAD" position ( figure 2 ), the analysis sample is directly injected through an autosampler, and pure methanol is used to rinse the octadecyl-bonded silica gel column A, so that the coenzyme Q10 in the plasma is retained on the column A;
[0028] 3) Continue to rinse column A with pure methanol, and some impurities remaining in the plasma will flow through column A into the waste liquid discharge line waste liquid bottle;
[0029] 4) When the six-way valve is in the...
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