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Microbial bacteriocide, and its preparing method and use

A bactericide and microorganism technology, applied in the field of microbial bactericide and its preparation, can solve problems such as quality decline and sharp drop in output

Inactive Publication Date: 2005-07-20
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All plants infected with the above two pathogenic bacteria not only have a sharp drop in yield, but also have a significant decline in quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Inoculate Brevibacillus laterosporus G4 cells on the slant of test tube agar medium. The medium formula is: 3 grams of beef extract; 8 grams of peptone; 4 grams of sodium chloride; 18 grams of agar; 1000ml of water, pH7.5; at 32 ° C Cultivate for 2 days to obtain test tube seeds;

[0034] Use a 500ml triangular flask to expand the culture, and the formula of the liquid medium is: 0.3% beef extract; 15% soybean meal; 1% fish meal; 0.4% sodium chloride; pH7.5; the rest is water. Fill each Erlenmeyer flask with 300ml of liquid culture medium, sterilize at 120°C for 30 minutes, put it into a test tube with a size of 1 square ml after cooling, and cultivate it at 37°C for 2 days at 120rpm / min, when the number of viable bacteria proliferates to The highest peak, reaching 10 10 / ml, it becomes an available microbial fungicide.

Embodiment 2

[0036] Basically the same as embodiment one, the difference is: the liquid medium formula is: 0.2% beef extract, 10% soybean meal, 0.5% fish meal, sodium chloride 0.3%, pH7, the remainder is water; 35°C, and the incubation time is 1.5 days.

Embodiment 3

[0038] Basically with embodiment one, difference is: liquid medium formula is: 0.4% beef extract, 20% soybean meal, 2% fishmeal, sodium chloride 0.5%, pH8, all the other are water; 38°C, and the incubation time was 1 day.

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Abstract

The present invention relates to a microbial bacteriocide, its preparation method and application. Said invented production strain is Brevibacillus laterosporus G4. Said invented microbial bacteriocide is prepared by adopting conventional shake-flask fermentation process, and its solid culture medium formula includes beef extract 3g, peptone 8g, sodium chloride 4g, agar 18g and water 1000 ml, pH 7.5 and its liquid fermentation culture medium formula contains 0.2-0.4% of beef extract, 10-20% of soybean care powder, 0.5-2% of fish powder, 0.3-0.5% of sodium chloride, pH 7-8 and the rest is water. Said invention has good bactericidal action for two plant pathogenic bacteria of fusarium and rhizoctonia.

Description

Technical field: [0001] The invention relates to a microbial bactericide and its preparation method and application, belonging to the technical field of microbial pesticides. Background technique: [0002] China is a large agricultural country, and the prevention and control of diseases, insect pests and weeds is an important link in agricultural production. At present, the control of agricultural and plant diseases still mainly relies on chemical fungicides. However, with the development of science and technology, studies have found that chemical pesticides are not only preventing and controlling diseases, but also polluting the environment more and more seriously. At the same time, chemical pesticides are often highly toxic, killing natural enemies, destroying biodiversity, and providing conditions for more serious pests and diseases to occur rampantly, resulting in a vicious circle in pest control. Pesticide residues caused by continuous drug use are harmful to the huma...

Claims

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Application Information

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IPC IPC(8): A01N63/02
Inventor 张楹周薇
Owner YUNNAN UNIV
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