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Diagnostic test for determining the concentration of transient proteolytic activity in composite biological media

A proteolytic, active technology for use in diagnostics

Inactive Publication Date: 2005-08-03
SYNAPSE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Problem B can be bypassed by using a fluorescent substrate, but it raises problem C

Method used

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  • Diagnostic test for determining the concentration of transient proteolytic activity in composite biological media
  • Diagnostic test for determining the concentration of transient proteolytic activity in composite biological media
  • Diagnostic test for determining the concentration of transient proteolytic activity in composite biological media

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Embodiment Construction

[0088] In order to practice the present invention, a typical description of the hemostatic and thrombosis system (HTS) will be given below, focusing on its most important enzyme, thrombin. It should be noted, however, that the present invention also relates to other enzymes and other physiological systems, such as other activated proteolytic enzymes (factors) of the coagulation system in blood and plasma, plasmin and other activators of the hemolytic fibrinolytic system in blood and plasma Components, complement factors activated in blood and plasma, pepsin in gastric juice, trypsin and chymotrypsin in duodenal juice, etc.

[0089] Typically, transient enzyme activity can be measured by adding a substrate that produces a signal upon transition, as described above. Where fluorogenic substrates are employed in the art, the method typically involves adding the fluorogenic substrate to blood (or another biological sample) in which thrombin production has been triggered (using meth...

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Abstract

A method is provided for determining in real time the course of thrombin activity in a sample of blood or plasma as it appears in and disappears from the simple which comprises adding a thrombin substrate to the sample that, per unit time, produces a detectable signal in a quantity that bears relation to the amount of thrombin present. Simultaneously, in a control sample of the same blood or plasma in which thrombin generation is not triggered, the activity of a standard preparation with invariable thrombin activity is measured. The exact molar amount of thrombin present at any moment is obtained by comparison of the activity measured in clotting blood and the simultaneously measured calibrator. The method is useful inter alia for diagnosing hyper- and hypo-coaguable states, either congenital, acquired or drug-induced in humans and animals. Also provided is a kit for use in this method.

Description

field of invention [0001] The invention belongs to the field of diagnosis, and more specifically relates to a method for real-time determination of the concentration course of biologically active enzymes instantaneously present in blood or other body fluids, and also relates to a test kit for implementing the method. Background of the invention [0002] a.Introduction [0003] The production and breakdown of proteolytic enzymes in body fluids is a key factor in many processes such as digestion, inflammation, blood coagulation, and thrombus formation. To give an example: Thrombin is an enzyme present transiently in blood coagulation and is a key enzyme in hemostasis and thrombus formation. Disorders of the hemostatic-thrombotic system (HTS) are critical in more than half of all disabling and fatal diseases. The less quantitatively important diseases, hemophilia and pulmonary embolism, are easily noticed. What is not widely recognized is that blood clots in the arteries ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01R33/465C12Q1/56G01N33/86
CPCG01N33/86C12Q1/56
Inventor 彼得·吉森亨德里克·C·赫姆克尔雷德·奥·迪耶里叙泽特·L·贝甘罗伯特·瓦根伍尔德
Owner SYNAPSE INC
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