Soy protein concentrate with low non-digestible oligosaccharides and process for its production
A technology for soybean protein and concentrate, which is applied in biochemical equipment and methods, protein composition of vegetable seeds, and vegetable protein processing, etc., can solve the problems of complex processing of isolates, high cost of isolates, and high sodium content.
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[0027] The present invention provides a process for the preparation of a protein concentrate comprising the steps of: providing a substantially defatted soy material; treating the material with an enzyme at an effective temperature and pH for an effective time; removing from the material either before or after the enzyme treatment fiber; inactivating enzymes after this treatment; and reducing the amount of carbohydrates by ultrafiltration so that the non-digestible oligosaccharides in the concentrate are less than 4.0% by weight of the total dry matter and the protein in the concentrate is at least 65.0% by weight of substance. The concentrate is then used in liquid or dry beverages, food or nutritional products.
[0028]In general, the method of the present invention includes: 1) dehulling all soybeans; 2) pressing the dehulled soybeans into flakes; 3) extracting soybean oil from the soybean flakes with a suitable solvent, such as hexane; Removal of solvent from defatted soy...
Embodiment 1
[0061] At 60°C, add 261.7 kg (577 pounds (lbs.)) of water to the mixing tank. Add 22.7 kg (50 lbs.) white soybean flakes. The pH was adjusted to 6.0 with hydrochloric acid. Add 22.7 grams (g) of Validase AGS enzyme. The slurry was mixed for 2 hours at 60°C. The pH of the enzyme-treated slurry was adjusted to 7.0 with 5% sodium hydroxide. The enzyme-treated, pH-adjusted slurry was injected into a Sharpless screw centrifuge at a rate of approximately 7.6 L per minute (2 gallons per minute, GMP). The liquid was jet cooked at 121.0°C. The jet cooked liquid was injected into an ultrafiltration membrane system with a 10000MWCO membrane. 25% of the original injected volume was removed as exudate. The retentate from the membrane system was spray dried with a high pressure pump feeding the nozzles. The spray-dried powder was subjected to sugar analysis by the method of Shukla, Fett Wissenschaft Technoligie, 89(2), pp. 75-79 (1987).
[0062] The soy protein concentrate contained...
Embodiment 2
[0064] At 60°C, 176.9 kg (390 pounds (lbs.)) of water was added to the mixing tank. Add 22.7 kg (50 lbs.) white soybean flakes. The pH was adjusted to 6.0 with hydrochloric acid. Add 22.7 grams (g) of Validase AGS enzyme. The slurry was mixed for 2 hours at 60°C. The pH of the enzyme-treated slurry was adjusted to 7.0 with 5% sodium hydroxide. Add 84.8 kg (187 lbs.) of water preheated to 60°C. The enzyme-treated, pH-adjusted slurry was injected into a Sharpless screw centrifuge at a rate of 2 gallons per minute (GPM). The liquid was jet cooked at 121.0°C. The jet cooked liquid was injected into an ultrafiltration membrane system with a 10000MWCO membrane. 75% of the original injected volume was removed as exudate. The retentate from the membrane system was spray dried with a high pressure pump feeding the nozzles. The dried product was analyzed to determine its content. The analysis results are shown in Table 1. All results are based on results without moisture unles...
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