Injectable chondrocyte implant

A technology of chondrocytes and compositions, applied in bone implants, joint implants, joint implants, etc.

Inactive Publication Date: 2005-09-07
VERIGEN AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They found that although morphological changes occurred in monolayer cultures compared to agarose gels, suspension cultures on alginate beads, or spinner cultures (where round cell morphology was maintained) (Aulthouse, A. et al., In Vitro Cell Dev. Biol, 1989, 25, 659; Archer, C. et al., J. Cell Sci. 1990, m97, 361; Haanselmann, H. et al., J. Cell Sci. 1994, 107, 17; Bonaventure, J. et al. , Exp.Cell Res.1994, 212, 97), however, expressed markers such as collagen types II and IX and large aggregated proteoglycans, aggrecan, versican and connexins were not altered

Method used

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  • Injectable chondrocyte implant
  • Injectable chondrocyte implant
  • Injectable chondrocyte implant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 - Chondrocyte Collection and Culture

[0089] By adding 2.5 ml of gentamicin (gentomycin) sulfate (concentration 70 micromol / liter), 4.0 milliliters of amphotericin (concentration 2.2 micromol / liter; trade name Fungizone®, an antifungal agent obtained from Squibb) , 15 milliliters of 1-ascorbic acid (300 micromol / liter), 100 milliliters of fetal calf serum (final concentration 20%), and the rest of the DMEM / F12 medium were mixed together to produce about 400 milliliters of growth medium, to prepare to transfer and / or process cartilage biopsies during culture and the growth medium used to culture chondrocytes (hereinafter "growth medium"). (The same growth medium is also used to transfer cartilage biopsies from the hospital to the laboratory, where the chondrocytes are extracted and propagated).

[0090] For autologous implants, cartilage biopsies are first collected by arthroscopic techniques, eg, from non-weight-bearing areas of the patient's joint, and tran...

Embodiment 2

[0096] Example 2 - Implantable Compositions

[0097] A carrier material of choice, such as a biocompatible, absorbable bead, mesh or thread, is mixed into transplantation medium in a sterile petri dish, the carrier material is "wetted" with the transplantation medium, and in one embodiment, The carrier material is exposed to the transplant medium for 1-10 hours or more. Chondrocytes are then added to the carrier material transplant medium mixture. Transplant medium can be 20% minimal essential medium with HAM F12 and 15 mM Hepes buffer and 10 to 20% autologous serum, all maintained at 37 °C in CO 2 in the incubator.

[0098] The chondrocytes are then contacted and grown on or in the carrier material for a period of time ranging from 1 hour to 1 week, and in one embodiment the chondrocytes are maintained at a temperature of about 37°C. Preferably, the chondrocytes are incubated overnight with the carrier material. In one embodiment, the chondrocytes and medium are gently ag...

Embodiment 3

[0102] Example 3 - Implantable Compositions

[0103] In another embodiment, chondrocytes suspended in culture medium can be added directly to the carrier material without "pre-wetting" the carrier material. In this case, the chondrocytes are then allowed to attach to and grow on or in the carrier material for a period of time ranging from about 1 hour to about 6 weeks. Preferably, the chondrocytes are incubated overnight with the carrier material. In one embodiment, the chondrocytes and medium are gently agitated so that the chondrocytes adhere to and grow on all sides of the carrier material.

[0104] In another embodiment, additional support material (the same or different than the original support material) is added during the culture to expand the cell culture. In another embodiment, the carrier material is first disrupted by using an enzyme such as trypsin. Additional support material having a surface area greater than the destroyed original support material is then ad...

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Abstract

The present invention relates to a flowable implantable composition comprising a support material, such as various forms of collagen or alginate beads or threads, which can support the attachment and growth of chondrocyte cells thereto, ad a method of making a flowable implantable composition comprising a support material and chondrocytes retained thereon. Further, the present invention relates to a method for the effective treatment of articulating joint surface cartilage by the transplantation of a flowable composition including a support material and chondrocyte cells retained thereon.

Description

field of invention [0001] The invention relates to the fields of chondrocyte cell implantation, cartilage transplantation, treatment, joint repair and prevention of arthritic lesions. In particular, the invention relates to a new form of implant and to a new method of chondrocyte cell implantation and cartilage regeneration. Background of the invention [0002] More than 500,000 arthroplasty surgeries and total joint replacements are performed annually in the United States. About the same number of similar procedures are performed in Europe. These numbers include about 90,000 total knee replacements and about 50,000 repairs of knee defects per year (see: Praemer A., ​​Furner S., Rice, D.P., Musculoskeletal conditions in the United States, Park Ridge, Ill.: American Academy of Orthopedic Surgeons, 1992, 125). Cartilage regenerative treatment methods are most useful and can be performed in the early stages of joint damage, thus reducing the number of patients requiring arti...

Claims

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Application Information

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IPC IPC(8): C12N11/02A61FA61F2/28A61F2/30A61KA61K45/00A61L27/00A61L27/38C12N5/07C12N5/077
CPCA61L27/3817A61L2430/06A61L27/3852A61F2/30A61F2/28
Inventor B·M·加内蒂V·古奈特
Owner VERIGEN AG
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