Outside sprout-cultivating-bottle radication method for saussurea involucrate set

Abstract

The invention relates to a method of Xinjiang saussurea involucrate group culture sprout bottle ectotrophic root, it comprises the following steps: match the MS or B5 or White or Nitsh or N6 well in the common condition without sterilization, mix them with supporting agent vermiculite or cellulose or chip or coconut furfuryl, separately load into the incubator, then wash the unrooted saussurea involucrate group culture sprout from the gelose with water, move the sprout into the incubator, semi-open culture for 10 to 15 days, then the saussurea involucrate sprout can arise good roots. The invention combines the arising root and growing seed as the first step, the gained saussurea involucrate root is well growled without exercise and domestication, it shortens the time, saves a good deal of cost, and is good to spread in mass production.

Description

technical field [0001] The invention relates to a method for rooting out of a bottle of snow lotus tissue culture seedlings Background technique [0002] Snow lotus (Saussurea involucrata.et kir) is one of the famous and precious Chinese herbal medicines, and Xinjiang is one of the main producing areas. At present, the snow lotus on the market mainly comes from the wild, which greatly destroys the fragile ecological environment; many areas in Xinjiang are suitable for the growth of snow lotus, so in recent years, the artificial cultivation of snow lotus has been carried out on a large scale, and the tissue culture technology is in the snow lotus. The role of large-scale artificial planting is increasingly valued. [0003] According to the relevant literature on the tissue culture of Xinjiang Saussurea, there are currently the following reports: [0004] W. Gubanova et al. used different parts of the young Xinjiang snow lotus plants as explants, inoc...

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Problems solved by technology

[0009] 1. Due to the presence of polysaccharides, the medium, containers and internal environment are polluted, which need to be sterilized by autoclaving and other methods. During the transplantation or subculture of plant tissues, the workbench and the like should be cleaned very carefully. operation, and then to avoid contamination as much as possible, such a process consumes a lot of labor and takes a long time, so this method has more problems

[0010] 2. In most cases, there is more water in the gel, and the induced roots are transplanted into the soil and the seedlings are prone to water loss, slow growth, and root growth delay, and because the medium (gel) is a non-gas phase, The obtained roots are rooted in water, and generally cannot be transplanted into the soil, and need to be hardened, and the survival rate of transplanting is low;

[0011] 3. Because most of the polysaccharides in the carbon source medium required for growth have a relatively low dependence on photosynthesis, so the growth of leaves is less, and it takes a long time to adapt after transplanting into the soil, and the resulting regeneration A lengthy, labor-intensive domestication step is essential until the plant becomes photoautotrophic; in addition, plants that cannot adapt to such domestication are prone to death

[0014] However, in the above-mentioned methods, due to the use of fungicides in the cultivation process, the plants are obviously damaged and the growth of the plants is delayed. This problem also greatly limits the application of these methods.

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