Human blood high density lipoprotein and its preparation method and use

A technology of high-density lipoprotein and manufacturing method, applied in the field of biochemistry, can solve the problems of underutilization and waste, and achieve the effect of significant economic benefits

Active Publication Date: 2005-11-23
江永忠 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, most blood products at home and abroad adopt Cohn’s low-temperature ethanol method to produce human serum albumin and gamma globulin. This method can divide plasma protein into five components such as I, II, III, IV and V during the separation process. Among them, the HDL-rich fraction IV-1 (FIV-1) is currently used as waste treatment and has not been utilized, which is actually a huge waste

Method used

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  • Human blood high density lipoprotein and its preparation method and use
  • Human blood high density lipoprotein and its preparation method and use
  • Human blood high density lipoprotein and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Take 1000L of raw plasma, add 190.5L of 50% ethanol, adjust the pH value to 7.1, protein concentration to 5.6%, and ionic strength to 0.15, stir at -2°C for 2 hours, let stand for 8 hours, use a 142-type continuous centrifuge, Centrifuge at 14000r / min to obtain 1190 L of supernatant and 12Kg of precipitate;

[0068]Add 5000 mL of HAC / NaAC mixed solution with a pH of 4.0 dropwise to the supernatant to make the pH of the mixed solution 6.90, add 263 L of 95% ethanol to make the protein concentration 3.4%, and the ionic strength 0.09, stir at -4°C for 2 hours, and statically Set aside for 8 hours, use a 142-type continuous centrifuge, and centrifuge at 14000r / min to obtain a supernatant of 1450L and a precipitate of 40Kg;

[0069] Add 682 L of water for injection to the supernatant, dropwise add 14.8 L of HAC / NaAC mixed solution with a pH of 4.0, so that the pH is 4.9, the protein concentration is 1.7%, and the ionic strength is 0.15. Place for 2 hours, use a 142-type con...

Embodiment 2

[0077] Take 1000L of raw plasma, add 190.5L of 50% ethanol, adjust the pH value to 7.3, protein concentration to 5.2%, and ionic strength to 0.18. Stir at -2°C for 2 hours, let stand for 8 hours, and use a 142-type continuous centrifuge. Centrifuge at 14000r / min to get supernatant 1100L and precipitate 12Kg;

[0078] Add 5000 mL of HAC / NaAC mixed solution with a pH of 4.0 dropwise to the supernatant to make the pH of the mixed solution 6.90, add 243 L of 95% ethanol to make the protein concentration 4.0%, and the ionic strength 0.15, stir at -6°C for 2 hours, and statically Set aside for 8 hours, use a 142-type continuous centrifuge, and centrifuge at 14000r / min to obtain a supernatant of 1449L and a precipitate of 39Kg;

[0079] Add 682 L of water for injection to the supernatant, dropwise add 14.8 L of HAC / NaAC mixed solution with a pH of 4.0, so that the pH is 5.0, the protein concentration is 2.0%, and the ionic strength is 0.09. Place for 2 hours, use a 142-type continuo...

Embodiment 3

[0087] Take 1000L of raw plasma, add 190.5L of 50% ethanol, adjust the pH value to 6.8, protein concentration to 4.0%, and ionic strength to 0.10, stir at -2°C for 2 hours, let stand for 6 hours, use a 142-type continuous centrifuge, Centrifuge at 14000r / min to obtain 1190L of supernatant and 13Kg of precipitate;

[0088] Add dropwise 5000 mL of HAC / NaAC mixed solution with a pH of 4.0 to the supernatant to make the pH of the mixed solution 6.90, add 263 L of 95% ethanol to make the protein concentration 2.0%, ionic strength 0.05, stir at -6°C for 2 hours, statically Set aside for 6 hours, use a 142-type continuous centrifuge, and centrifuge at 14000r / min to obtain a supernatant of 1450L and a precipitate of 38Kg;

[0089] Add 658 L of water for injection to the supernatant, dropwise add 14.3 L of HAC / NaAC mixed solution with a pH of 4.0, so that the pH is 4.5, the protein concentration is 1.0%, and the ionic strength is 0.05. Stir at -3°C for 2 hours, and statically Place fo...

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Abstract

The invention relates to a human high density lipoprotein and product, preparation process and use thereof, which comprises producing human plasma albumin and gamma globulin by employing low-temperature ethanol method, using discarded FIV-1 deposition as raw material, extracting HDL from FIV-1 through separation and purification steps. The prepared human high density lipoprotein and product can be applied for the preparation of medicament for treating multiple organ disturbance syndrome (MODS) and cardiovascular diseases such as atherosclerosis (AS).

Description

technical field [0001] The invention relates to a human blood high-density lipoprotein product, its manufacturing method and its application in pharmacy, belonging to the field of biochemistry. Background technique [0002] AS is a common multiple disease characterized by thickening and hardening of the arterial wall, loss of elasticity, and formation of atherosclerotic lesions due to lipid deposition, fiber and connective tissue proliferation in the arterial wall. Arterial lumen occlusion caused by AS can cause insufficient blood supply to vital organs such as the heart, brain, and kidneys, thereby seriously threatening human health and life. At present, the commonly used drugs for the treatment of AS clinically generally include vasodilator drugs, blood lipid-lowering drugs, antiplatelet drugs and thrombolytic drugs, etc. Although these drugs can fight against AS by relieving vasomotor disturbance and regulating blood lipids, none of them can prevent and eliminate AS plaq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47
Inventor 江永忠陈爱民周潮樊绍文
Owner 江永忠
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