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Immune adsorption column used for blood purification treatment

An immunoadsorption and blood purification technology, which is used in solid adsorbent liquid separation, blood filtration, chemical instruments and methods, etc. Stability, good application prospects, the effect of improving chemical and physical stability

Inactive Publication Date: 2006-01-11
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since protein A is a biological macromolecule, it is easy to denature and inactivate and is expensive, so the preparation and storage costs of protein A immunoadsorption materials are high, and the service life of adsorption materials is short
At the same time, it increases the cost of treatment for patients

Method used

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  • Immune adsorption column used for blood purification treatment
  • Immune adsorption column used for blood purification treatment
  • Immune adsorption column used for blood purification treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1. Preparation of L-histidine immunoadsorption medium by direct bonding method

[0019] (1) Preparation of epoxy medium synthesis

[0020] Measure 15mL of Sepharose CL-4B gel with a graduated cylinder, add 18mL of water, 7mL of 2mol / L NaOH, and 5mL of epichlorohydrin, and mix well. React at 40°C for 2 hours. Stir during the reaction. After the reaction is complete, rinse with absolute ethanol and water, and filter to dry.

[0021] (2) Preparation of L-histidine affinity adsorption medium

[0022] in 30mL NaHCO 3 1.5 mmol L-histidine was added to the buffer, and the pH was adjusted to 13 with NaOH. Add the solution of epoxy Sepharose CL-4B gel and histidine, mix well, and react at 60°C for 24 hours. After the reaction is completed, rinse with absolute ethanol and water respectively, and filter to dry.

[0023] The binding amount of L-histidine was 12.7 mg / g, and the adsorption amount to human IgG (molecular weight: 160 kD) was 12.0 mg / g.

[0024] The schemat...

Embodiment 2

[0025] Example 2 Carbonyl Diimidazole Covalently Bonded L-Histidine Immunoadsorption Medium

[0026] (1) Synthesis of epoxy medium

[0027] Measure 15mL of Sepharose CL-4B gel with a graduated cylinder, add 18mL of water, 7mL of 2mol / L NaOH, and 5mL of epichlorohydrin, and mix well. React at 40°C for 2 hours. Stir during the reaction. After the reaction is complete, rinse with absolute ethanol and water, and filter to dry.

[0028] (2) Synthesis of amino mediator

[0029] Mix the epoxy gel prepared above with 5mL water, 45mL 13g / L hexamethylenediamine-NaHCO 3 The buffer solution was mixed, and NaOH was used to adjust the pH to 11. The reaction was carried out at 80°C for 24 hours, and stirring was required during the reaction. After the reaction was completed, wash with absolute ethanol and water, respectively, and filter to dry.

[0030] (3) Synthesis of L-histidine affinity adsorption medium

[0031] Take a certain volume of dioxane in a round bottom flask, add appropri...

Embodiment 3

[0035] Example 3 Preparation of protein A immunoadsorption medium

[0036] (1) Synthesis of epoxy medium

[0037] Measure 15mL of Sepharose CL-4B gel with a graduated cylinder, add 18mL of water, 7mL of 2mol / L NaOH, and 5mL of epichlorohydrin, and mix well. React at 40°C for 2 hours. Stir during the reaction. After the reaction is complete, rinse with absolute ethanol and water, and filter to dry.

[0038] (2) Synthesis of amino medium:

[0039] Mix the above epoxy gel with 5mL water and 45mL 13g / L hexamethylenediamine-NaHCO3 buffer. React in a shaker at 50-55°C for 2 hours. Stir during the reaction. After the reaction is complete, rinse with absolute ethanol and water, and filter to dry.

[0040] (3) Synthesis of aldehyde-based medium

[0041] Mix the amino gel above with 37 mL of water and 3 mL of 25% glutaraldehyde. React at room temperature for 2 hours. Stir during the reaction. After the reaction is completed, wash with water, 2mol / L acetic acid and water respectivel...

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Abstract

An immunoadsorption column for blood cleaning therapy is prepared through proportionally mixing protein A and L-histidine ligand, homogenizing and filling the mixture in column. Its advantages are high adsorption power to IgG, high stability and long service life.

Description

Technical field: [0001] The invention relates to blood purification treatment technology, and in particular provides an immunoadsorption column for blood purification treatment. Background technique: [0002] Blood immunoadsorption therapy is an emerging clinical medical technology in recent years. It is used for some difficult diseases that have no obvious curative effect on drugs, such as autoimmune system diseases, organ transplant rejection, severe hepatitis, malignant tumors, nephrotic syndrome, jaundice, etc. In vitro immunoadsorption blood purification can often achieve good results. Blood immunoadsorption therapy technology is being widely used in various clinical fields such as urology, liver disease, blood, and heart failure. The development and production of blood immunoadsorption column has also become an important high-tech industry. [0003] Protein A is a single-peptide chain protein produced by Staphylococcus aureus, which has a broad binding ability to imm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M1/34B01D15/00
Inventor 邹汉法孔亮刘月启唐守万
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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