Method of preparing natural human thymosin a1 using series expression mode

Abstract

The invention discloses a method to manufacture biology polypeptide, especially a method to make natural human thymosin by gene series express method. It contains compounding two polynucleotide section, compounding double enzyme cut, T alpha 1 gene three times connecting in series, constructing high efficiency expression, constructing engineering fungus, expressing T alpha 1 polypeptide six series bodies in engineering fungus, purifying and cracking. The invention could abundantly express aim albumen, and it has simple technology, easy to operate and low cost.

Description

technical field

[0001] The invention relates to a method for preparing biological polypeptides, in particular to a method for preparing natural human thymosin α1 by means of gene serial expression. Background technique

[0002] Thymosin α1 (Thymosin-α1, Tα1) is a polypeptide composed of 28 amino acid residues, with an isoelectric point of 4.2, no secondary structure and no disulfide bond, and the only modification is N-terminal acetylation. Its amino acid sequence is N-Ac-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu- Val-Val-Glu-Glu-Ala-Glu-Asn-C is an immune enhancer targeting T lymphocytes. It can make T cells mature and differentiate, and can promote mature T cells and NK cells to secrete various lymphokines such as interleukin-2 and γ-interferon. -2 receptor (IL-2) production.

[0003] As an immune enhancer, Tα1 is mainly used for immunodeficiency and immunosuppressive diseases. When a patient is infected with certain viruses su...