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Simple silicon rubber membrane biological reaction bottle cultivation system for plant cell

A technology of membrane bioreaction and plant cells, which is applied in the field of simple silicon rubber membrane bioreactor culture system for plant cells, can solve the problems of high production cost, complicated design and production, unfavorable non-bubble film air supply culture method, etc., and achieve improvement Application surface, effect of improving application degree

Inactive Publication Date: 2006-02-22
SHANGHAI JIAO TONG UNIV
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  • Application Information

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Problems solved by technology

However, the design and production of the reaction tank and the device used to fix the silicone rubber tube are relatively complicated, and the production cost is relatively high, which is not conducive to the culture method of air supply without bubble film, which limits the culture that can effectively reduce the shear damage of bubbles. Application and promotion of the method in cell culture

Method used

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  • Simple silicon rubber membrane biological reaction bottle cultivation system for plant cell
  • Simple silicon rubber membrane biological reaction bottle cultivation system for plant cell
  • Simple silicon rubber membrane biological reaction bottle cultivation system for plant cell

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Embodiment Construction

[0016] Such as figure 1 , 2, 3, and 4, the present invention includes: an air pump 1, a gas filter 2, a temperature-controlled incubator 3, a gas flow meter 4, a gas humidifier 5, a silicone rubber membrane bioreactor 6, a silica gel gas delivery tube 7, Magnetic stirring bar 8, magnetic stirring device 9. The main body of the gas humidifying device 5 is a cylindrical hemispherical top glass container, the top of which is integrated with a first air inlet port 10 and a first gas outlet port 11, and the first air inlet port 10 extends into the cylindrical hemispherical top glass container near the bottom. Gas flow meter 4, gas humidification device 5, silicone rubber membrane biological reaction bottle 6, silicone gas delivery tube 7, magnetic stirrer 8, and magnetic stirrer 9 are all located inside the temperature-controlled incubator 3, and the magnetic stirrer 8 is placed in the silicone rubber The inner bottom of the membrane bioreactor 6, the magnetic stirrer 9 is located...

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Abstract

Disclosed is a plant cell simplified silicone rubber film biological reaction bottle culture system which comprises, an air pump, an air filter, a temperature control incubator, a gas flow meter, a gas humidifying apparatus, a silicone rubber film biological reaction bottle, a silica gel pneumatic tube, a magnetic stirring unit and a magnetic stirrer.

Description

technical field [0001] The invention relates to equipment in the field of biotechnology, in particular to a plant cell simple silicon rubber membrane biological reaction flask culture system. Background technique [0002] Plant cells can synthesize many valuable secondary metabolites, including medicinal active substances, food additives, pesticides, feed, fine chemical products, etc. The use of large-scale suspension culture of plant cells in bioreactors to produce useful metabolites of plants has made great progress in recent years. The design and development of reactors generally refer to microbial culture tanks. In the early days of this research field, even direct use of fermentation tanks The apparatus cultivates plant cells. However, plant cells are larger than microbial cells, and the diameter of a single cell is approximately between 20 and 100 μm. They are surrounded by a rigid fibrous substrate and usually have a large vacuole, which can occupy more than 95% of ...

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Application Information

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IPC IPC(8): C12M3/00C12M1/00
CPCC12M23/20C12M23/08
Inventor 陈思晔齐瀚实张宝红黄娜
Owner SHANGHAI JIAO TONG UNIV