Method for preparing transparent zinc hyaluronic acid
A technology of zinc hyaluronate and sodium hyaluronate, which is applied in the field of preparation of zinc hyaluronate, and can solve problems such as long production cycle, low yield and complex process
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Embodiment 1
[0015] Add 200 grams of sodium hyaluronate into 2 liters of alcohol solution containing 2% zinc acetate (45% alcohol), stir for 6 hours, let it stand, pour off the alcohol supernatant, and replace it 6 times in total. Dehydrate with 95% ethanol for 5 times until the alcohol content reaches 93%, centrifuge to remove the ethanol, and dry in a vacuum oven until the moisture content reaches 7%.
[0016] Test result: Viscosity: 21mm 2 / s, glucuronic acid content: 42.3%, zinc content: 8.9%, sodium content: 0.2%.
Embodiment 2
[0018] Take 190 grams of sodium hyaluronate, stir, add 2 liters of 95% methanol containing 3% zinc sulfate, and start stirring. After 8 hours, stop stirring, pour off the methanol supernatant, repeat the above steps, and replace 8 times in total. Dehydrate with analytical pure methanol for 3 times, centrifuge to remove methanol, and dry in a vacuum oven until the water content reaches 7%.
[0019] Test result: Viscosity: 19mm 2 / s, glucuronic acid content: 43.1%, zinc content 6.2%, sodium content: 2.7%.
Embodiment 3
[0021] Add 150 grams of sodium hyaluronate into 2 liters of 95% ethanol solution containing 5% zinc chloride, stir for 10 hours, let stand, pour off the alcohol supernatant, repeat the above steps, and replace 8 times in total, then use Analytical pure ethanol was dehydrated 5 times, centrifuged to remove alcohol, and dried in a vacuum oven until the water content reached 7%.
[0022] Test result: Viscosity: 23mm 2 / s, glucuronic acid content: 41.9%, zinc content 9.1%, sodium content: 0.2%.
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