Sterile immunogenic non-tumorigenic tumor cell compositions and methods

A tumor cell, immunogenic technology, applied in drug combinations, anti-tumor drugs, pharmaceutical formulations, etc., can solve problems such as changing immunogenicity, cell inactivation, and changing proteins

Inactive Publication Date: 2006-05-17
INTRACEL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although irradiation with about 1-3 MRads (Mrads) is sufficient to kill microorganisms, it will change the structure of proteins, DNA, RNA, etc., and either biologically change the expected immunogenicity or other biological functions of the cells or completely kill the cells. live

Method used

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  • Sterile immunogenic non-tumorigenic tumor cell compositions and methods
  • Sterile immunogenic non-tumorigenic tumor cell compositions and methods
  • Sterile immunogenic non-tumorigenic tumor cell compositions and methods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Colon and orthotopic tumor washing prior to dissection

[0041] Physiological saline (different from HBSS) for intravenous use is available in 500 mL soft bags. By aseptically fitting a sterile port (eg, a Combi-port) in the bag, the bag can essentially be converted into a spray bottle. By squeezing the bag, a stream of saline is forced directly onto the tumor and surrounding colonic mucosa.

[0042] For the first wash, the colon can be held vertically and washed with 500 mL of normal saline from the top down. In preliminary experiments, the second 500 mL wash consisted of saline or 1% Triton X-100 (dissolved in saline), and was directed at the tumor and its close adjacent areas. This washing is also done when the colon is held vertically. The colon is then held horizontally and the area of ​​the colon containing the tumor is elevated slightly. Tumors were then subjected to further washes (2-3 times), each containing 500 mL of saline. The fluid after e...

Embodiment 2

[0049] Chemical Disinfection of Colon Tumors Before Enzymatic Dissociation

[0050] For pathology stage purposes, pathologists cut tumors to determine the deepest margin of invasion. Therefore, the resected tumor contained several fragments of tumor tissue. The tissue was dissected to remove extraneous non-tumor and necrotic tissue. Trimmed tumor fragments were further subdivided for homogeneity and divided into 2-4 groups for processing according to the experimental design. The tissue was distributed as evenly as possible among the samples so that tumor fragments in the experimental groups looked the same as possible. Trimmed pieces were added to test tubes containing 40 mL of disinfectant and then processed by shaking at 200 rpm for 2 min on a rotary platform shaker. Trimmed tumor fragments were then washed 3 times with HBSS before dissociation. The amount of dissociated tumor was determined by weighing the tumor fragments before dissociation and after comple...

Embodiment 3

[0066] Role of the presence of antibiotics during colon tumor dissociation

[0067] Gut microbes trapped within invaginations in colon tumors are released during enzymatic dissociation. In addition, the enzymatic dissociation process occurs under conditions favorable to the growth of intestinal microorganisms (37 °C). For these reasons, the effect of adding antibiotics to resolvase solutions and other treatment solutions has been investigated.

[0068] Antibiotics are usually used for a longer period of time. The first set of experiments was designed to assess the efficacy of various antibiotics at shorter exposure times. The next set of experiments examined the effectiveness of the antibiotics when included in the resolvase solution.

[0069] Appropriate test microorganisms are incubated with antibiotics at 4°C or 37°C for various periods of time. "Untreated" controls were incubated with HBSS. After treatment, samples were assayed for bioburden by membrane filtrati...

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Abstract

This invention relates to methods of removing bioburden from an aggregate of cells to obtain sterile cells that remain viable and immunogenic for the production of vaccines. This invention further relates to a method of eliciting an immune response to prevent a recurrence of metastases that involves preparing and administering a sterile vaccine derived from solid tumors. The vaccine is prepared by excising a solid tumor from a cancer patient, digesting the tumor cells with an enzyme to obtain dissociated cells, irradiating the dissociated cells to render the cells non-tumorigenic, and sterilizing the cells.

Description

[0001] This application claims the benefit of US Provisional Application Serial No. 60 / 358,431, filed February 22, 2002, the entire contents of which are incorporated herein by reference. field of invention [0002] The present invention relates generally to methods of biological cell sterilization and the resulting sterile cell products; more particularly, but not by way of limitation, to cancer vaccines and methods for the preparation of sterile immunogenic and viable, but non-tumorigenic method of tumor cell composition. Background of the invention [0003] Sterilization of biological cell compositions for prophylactic and therapeutic purposes is difficult because the chemical, physical, or physiological properties of the cells may be significantly altered due to changes in the environment surrounding the cells. For example, gas sterilization using ethylene oxide is known to be toxic and carcinogenic. Although irradiation with about 1-3 MRads (Mrads) is sufficient to kil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61K39/00C12N15/85
CPCA61K39/0011A61K2039/5152A61K2039/55594A61P35/00A61P35/04
Inventor M·V·哈斯佩尔N·波马托M·G·小汉纳
Owner INTRACEL RESOURCES
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