Method for extracting mucus of S. gigantea used as antibiotic substance

Abstract

A process for extracting the antibacterial substance from the mucilage of S. gigantea includes such steps as thawing said mucilage, proportionally adding it to the extracting solvent prepared from water, Na4EDTA, phenylmethyl sulfuryl fluoride, aprotinin and acetic acid, stirring on ice bath to obtain liquid extract, centrifugal of supernatant, filtering, ultrafiltering, concentrating, and freeze drying.

Description

technical field

[0001] The invention relates to a method for extracting antimicrobial substances from the mucus of giant ligant coral (S. gigantea) Background technique

[0002] Coral reef ecosystems are among the most productive and diverse systems on Earth, however, in recent decades many scientists have invested in the disease resistance of stony corals as disease outbreaks have killed coral polyps and led to reef bleaching. researching. The mucus secreted by coral polyps covers the surface of the reefs formed by coral polyps. This layer of mucus is the first gateway for corals to resist pathogens. Therefore, it is very important to study the anti-bacteria effects of mucus. The compounds contained in the mucus not only act as the coral's own anti-infection substances, but the biological activity of many compounds can also provide antimicrobials for humans.

[0003] Although the study of mucus in stony corals is of great significance, the method of mucus extraction has r...