Methods and compositions for detecting SARS virus and other infectious agents

A technology of viruses and coronaviruses, applied in biochemical equipment and methods, measurement/testing of microorganisms, resistance to vector-borne diseases, etc., can solve the problem that RT-PCR cannot meet the needs of early clinical screening and diagnosis, RT-PCR detection Low rate, expensive and other issues

Inactive Publication Date: 2006-07-12
CAPITALBIO CORP +1
View PDF25 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, RT-PCR cannot exclude infected patients before SARS virus expression, and the detection rate of RT-PCR is very low
Detection process requires expensive real-time PCR equipment
Therefore, RT-PCR cannot meet the needs of early clinical screening and diagnosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods and compositions for detecting SARS virus and other infectious agents
  • Methods and compositions for detecting SARS virus and other infectious agents
  • Methods and compositions for detecting SARS virus and other infectious agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0227] Example 1. Probe design

[0228] Various genomic sequences of SARS-CoV are available (see Table 22 for example).

[0229] serial number

SARS crown

Virus

Source

Country of submission

Home (region)

GenBank

Acc

N in the sequence

Number of

Genome leader

Degree

Ratio of N

example

SARS_BJ01

Beijing China

China

AY278488

900

28920

3.11％

SARS_BJ02

Beijing China

China

AY278487

300

29430

1.02％

SARS_BJ03

Beijing China

China

AY278490

607

29291

2.07％

SARS_GZ01

Beijing China

China

AY278489

1007

29429

3.42％

SARS_BJ04

Beijing China

China

AY279354

2502

24774

10.10％

SARS_

CUHK-W1

China Hong Kong

China Hong Kong

AY278554

0

29736

0.00％

SARS_HKU-398

49

China Hong Kong

...

Embodiment 2

[0243] Example 2: Pre-processing of blood samples

[0244]The pre-processing of blood samples involves a relatively complicated process. However, considering the relatively low SARS virus concentration reported in serum, the pretreatment described here can effectively enrich lymphocytes from approximately 2 ml of whole blood in order to increase the chance of detection.

[0245] 1. Sample collection and transmission

[0246] 1) Collect samples from patients in the hospital and put them into the first transmission window. Then close and lock the door of the transmission window.

[0247] 2) Then transfer the sample to the second transmission window. Record the sample in the notebook and print 3 barcode labels. Then the sample is routinely tested and transferred to the pre-processing transmission window.

[0248] 2. Use a biological safety cabinet

[0249] 1) The hospital staff performing the pre-processing process enter the pre-processing studio and close the door. Open the bi...

Embodiment 3

[0277] Example 3. The process of using QIAamp Viral RNA kit to extract RNA

[0278] The following steps are used in the RNA preparation process:

[0279] 1. Use a pipette to add 560μl of the prepared buffer AVL containing Carrier RNA to a 1.5ml microcentrifuge tube. If the sample volume is greater than 140 μl, increase the amount of buffer AVL / Carrier RNA in the same proportion (for example, a 280 μl sample will require 1120 μl of buffer AVL / Carrier RNA).

[0280] 2. Add 140μl of plasma, serum, urine, cell culture supernatant or cell-free body fluid to the buffer AVL / Carrier RNA in the microcentrifuge tube. Mix for 15 seconds with a pulse vortex shaker. To ensure effective lysis, it is important to thoroughly mix the sample with buffer AVL to obtain a uniform solution. Frozen samples that have only been thawed once can also be used.

[0281] 3. Incubate for 10 minutes at room temperature (15-25°C). The virus particles can be completely lysed after 10 minutes at room temperature....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention relates generally to the field of virus detection. In particular, the invention provides chips, probes, primers, kits and methods for amplifying and detecting SARS-CoV nucleotides sequence. The clinical and other uses of the present chips, probes, primers, kits and methods are also contemplated.

Description

Background of the invention [0001] Since November 2002, a disease called Severe Acute Respiratory Syndrome (SARS) has been reported in 22 countries around the world. As of May 2, 2003, the WHO has reported a total of 6,054 SARS cases and 417 deaths among the infected people. At the same time, China reported a total of 3,788 SARS cases and 181 deaths among the infected population. [0002] The main symptoms of SARS patients include fever (above 38°C), headache and body aches. After the disease lasts for 2-7 days, the patient may develop a dry cough without sputum accompanied by breathing difficulties. [0003] Based on discoveries in Hong Kong, Canada and the United States, a previously unidentified coronavirus has been identified as the pathogen causing SARS. Researchers have discovered that SARS coronavirus is a positive-strand RNA virus that does not require intermediate steps in DNA during replication and uses standard codons (Marra et al., Science May 1, 2003; (before printing...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/70
CPCC12Q1/6837C12Q1/701C12Q1/686Y02A50/30
Inventor 李泽陶生策程京
Owner CAPITALBIO CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap