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Algae bioflocculation agent preparation method

A biological flocculant and flocculant technology are applied in the field of preparation of algal biological flocculants, which can solve the problems of different algae species and complicated processes, and achieve the effects of easy control, simple process and good flocculation effect.

Inactive Publication Date: 2006-07-26
HUAZHONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The algae species are different, and the process is more complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: cultivate aseptic Chlamydomonas, its culture medium formula is as follows:

[0027] NaNO 3 0.85g

[0028] MgSO 4 ·7H 2 O 0.37g

[0029] Na 2 SiO 3 9H 2 O 0.274g

[0030] CaCl 2 2H 2 O 0.04g

[0031] K 2 HPO 4 ·3H 2 O 0.04g

[0032] Na 2 CO 3 0.02g

[0033] Citric acid 6.0mg

[0034] Ferric Ammonium Citrate 6.0mg

[0035] EDTA 1.0mg

[0036] A-5 solution 1.0ml

[0037] Distilled water 1.0L

[0038] pH 7.4 at room temperature

[0039] Above-mentioned A-5 solution formula is as follows: H 3 BO 3 0.7g, MnCl 2 4H 2 O 1.81g, Na 2 MoO 4 ·H 2 O0.39g, ZnSO 4 ·7H 2 O 0.222g, CuSO 4 ·H2 O 0.196g, Co(NO 3 ) 2 ·6H 2 O 0.049g, distilled water 1.0L.

[0040] The culture conditions are temperature 25°C, light 45μEm -2 the s -1 . The extraction and preparation steps of the Chlamydomonas flocculant are as follows: when the algae is in a stable growth period, take the algae liquid and centrifuge at a speed of 4500g for 20 minutes. T...

Embodiment 2

[0041] Embodiment 2: Cultivate aseptic Chlamydomonas, its culture medium formula is the same as embodiment 1, culture condition is temperature 35 ℃, light 45 μ Em -2 the s -1 . The extraction and preparation method of Chlamydomonas flocculant is the same as in Example 1.

Embodiment 3

[0042] Embodiment 3: Cultivate aseptic Chlamydomonas, its culture medium formula is the same as embodiment 1, culture condition is temperature 15 ℃, light 45 μ Em -2 the s -1 . The extraction and preparation method of Chlamydomonas flocculant is the same as in Example 1.

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PUM

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Abstract

The invention provides a process for preparing biological flocculation agents of algae, which comprises culturing germ-free algae, charging anhydrous alcohol into the culture liquid for settlement, and finally concentrating to obtain the flocculating agent with effective flocculation results and good precipitability.

Description

technical field [0001] The invention relates to the treatment of domestic sewage, industrial wastewater, and the processing technology of food, beverage, brewing and other products, and more specifically relates to a preparation method of an algae bioflocculant. Background technique [0002] Inorganic flocculants represented by aluminum salts, iron salts and their polymers and synthetic organic polymer flocculants represented by polyacrylamide are commonly used flocculants at present. Because they cannot be degraded and have certain toxicity, the ecological environment has been affected. Serious pollution also poses a serious threat to human health (Duan and Gregory, 2003; Zhang and Lin, 1999; Zouboulis et al., 2004). Microbial flocculants are a class of biological macromolecules secreted by microorganisms with flocculation activity. Microorganisms that produce flocculants include algae, bacteria, fungi, and actinomycetes (Zhang and Lin, 1999; Zha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/54C12N1/12
Inventor 杨劭朱迟高健
Owner HUAZHONG NORMAL UNIV
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