Wickerhamiella domercqiae Y2A for producing sophorose lipid and its uses

A technology of Wykkimatous yeast and microbial fermentation biosurfactant, applied in the field of yeast, to achieve the effect of good cell apoptosis and promotion of cell apoptosis

Inactive Publication Date: 2006-07-26
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the strains used to ferment and produce sophorolipids in the world are limited to a few strains of yeast in the American Type Culture Collection, and the sophorolipid-producing strains with independent intellectual property rights in my country have not been reported before 2005

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Screening of Wickerhamiella domercqiae var. sophorolipid CGMCC No. 1576 strain.

[0033]From soil samples and water samples from Daqing Oilfield, Shengli Oilfield, Jinan Vegetable Oil Factory, Jinan Oil Refinery, Caishi Factory, Jinan Xiaoqing River sewage, domestic sewage, Guangzhou Shunde Oil Refinery and Food Factory, Anhui Rapeseed Field, through enrichment culture, More than 200 strains of yeast were screened by streaking on the plate. Subsequently, shake flask fermentation was carried out, and the oil-expelling activity and surface tension of the fermentation liquid were detected, and 2 strains of yeast were obtained from the rape leaf-derived sample. Fermentation was carried out under the conditions that the culture temperature was 30° C., the rotation speed of the shaker was 150 rpm, and the initial pH of the fermentation medium was pH 6.0. Among them, the fermentation product of Pseudomonas spp. has good oil-expelling activity, and can reduce the su...

Embodiment 2

[0038] Example 2: Physiological and biochemical characteristics of the strain Wickerhamiella domercqiae var. sophorolipid CGMCC No. 1576.

[0039] Wickerhamiella domercqiae

[0040] According to the morphological observation and physiological and biochemical experiments, the screened sophorolipid-producing bacterial strain of the present invention is determined to be a strain of the genus Pseudomonas spp. Check the literature related to the physiological and biochemical characteristics of Pseudomonas spp., only relate to the report of Pseudomonas spp. producing sophorolipid biosurfactant in the literature published by the inventor, but the sophorolipid output is relatively low (about 40g / L ), thus this high-yield sophorolipid-producing Pseudomonas spp. belongs to Pseudomonas spp. variety Wickerhamiella domercqiae var.sophorolipid.

Embodiment 3

[0041] Example 3: Preparation of sophorolipid biosurfactant by using Wickerhamiella domercqiae var. sophorolipid CGMCC No.1576. Wherein, the fermentation temperature is 25°C.

[0042] (1) Strain selection: Wickerhamiella domercqiae var. sophorolipid CGMCCNo.1576;

[0043] (2) Slant culture: Inoculate the strains on a basic inorganic salt medium, add agar with a mass volume ratio of 1.6%, and culture statically for 24 hours at 25°C;

[0044] (3) Seed culture: the bacterial strain cultivated in step (2), under aseptic conditions, connect 1 to 2 loops with an inoculation loop in 20 mL of a medium containing 4% glucose and 1% rapeseed oil in a mass volume ratio , under the condition of 25°C, shake culture for 24 hours to obtain seed solution;

[0045] (4) Expanded cultivation: with an inoculum amount of 5% by volume, the seed solution was inoculated in 100 mL of a medium containing rapeseed oil with a mass volume ratio of 1%, and cultured with shaking for 96 hours at 25°C;

[0...

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Abstract

This invention discloses a quasi-Wicker yeast variety Wickerhamiella domercqiae var. sophorolipid. This strain is conserved in general microbe center in China microbe bacterial conservention and management committee (institute of microbe of Chinese Academy of Sciences, Beijing, China), and its preservation number is CGMCC No. 1576. This invention also discloses the appliance of the quasi-Wicker yeast variety in preparing sophorosid biosurfactant that can supress neoplasms outside of body. The sophorosid is prepared through following steps: (1) chamfer culture(2)seed culture(3) enlargement culture(4) fermentate collection(5)production separation, and others. This method has many advantanges, for example, it is easy to operate; the output of sophorosid is high; the generated sophorosid has high restraining rate to tumor cell...so it has very broad application prospect in drug field.

Description

technical field [0001] The invention relates to a strain of sophorolipid-producing yeast and its application, specifically, a strain of sophorolipid-producing yeast strain of Wickerhamiella domercqiae var. sophorolipid and its application. Background technique [0002] Sophorolipid is an important biosurfactant. The research on it began in the 1950s and 1960s, and it was mainly obtained through microbial fermentation. Compared with chemically synthesized surfactants, it has many advantages such as excellent surface properties and environmental friendliness, and because the raw materials for the synthesis of sophorolipids are mainly some cheap oils and sugars, and it is non-toxic, odorless and tasteless , has no stimulating effect on human skin, so sophorolipids are mainly used in many fields such as petroleum, metallurgy, cosmetics, food, and medicine to reduce surface tension, stabilize emulsions and increase foaming. [0003] In recent years, foreign research on sophoroli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02
Inventor 宋欣曲音波
Owner SHANDONG UNIV
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