Liver support method of novel external biological artificial liver

Abstract

The present invention relates to method of improving oxygen supply for biological artificial liver cell. The blood plasma after separation is first oxygenated in the concentrated erythrocyte plasma tank, treated in the bioreactor to extract, convert and metabolize toxic matter and secrete great amount of bioactive matter to the plasma, and separated from the mixed erythrocyte liquid to obtain concentrated erythrocyte component; and the plasma detoxicated and replenished with bioactive matter and the concentrated erythrocyte component flow back to the plasma tank to complete the bioreactor circulation and separate out plasma with low toxicity and containing bioactive matter. The method is superior to available biological artificial support method, which has insufficient oxygen supply, serious rejection and other demerits. The method has specific combination of erythrocyte with oxygen to raise the oxygen content in liquid greatly, provides bioactive cell with excellent survival condition, and is favorable to oxygen ingestion of liver cell.

Description

technical field [0001] The invention relates to a bioartificial liver, in particular to a method for improving the oxygen supply of bioartificial liver cells. Background technique [0002] Currently, liver support technologies for existing artificial livers include mechanical artificial livers and bioartificial livers. The mechanical artificial liver uses mechanical principles such as hemodialysis, filtration, and adsorption to remove toxic components in the blood to replace part of the detoxification function of the liver. However, in addition to the detoxification function of the liver, it also has the function of synthesizing proteins and other biologically active substances, as well as regulating metabolism in the body, and these functions cannot be solved by using a mechanical artificial liver. The bioartificial liver uses bioactive cells with partial or complete liver cell activity to enter the bioreactor, and the blood or some of its components flow through the biore...

AI Technical Summary

Problems solved by technology

Firstly, the physical dissolution rate of oxygen in plasma is extremely low, only 0.0031mL / 100mL / mmHg. Secondly, the oxygen content of simple plasma has a linear relationship with the oxygen partial pressure, that is, the oxygen partial pressure also increases at the same time when the oxygen content is higher. High, and the drop of oxygen content will lead to a straight drop in oxygen partial pressure. Specifically, in the bioreactor, the cells at the front end of perfusion will always be exposed to high oxygen partial pressure, and the continued high oxygen partial pressure will have a certain impact on the cells. Toxicity, with the continuous intake of oxygen, the partial pressure of oxygen drops rapidly. After perfusion, some cells will not be able to absorb enough oxygen from the liquid due to the low partial pressure of oxygen and will be in a state of hypoxia

Of course, some bioartificial livers use blood to perfuse the bioreactor directly, and the red blood cells in the blood can increase oxygen for the bioactive cells. C3A) and heterogeneous liver cells (such as pig liver cells), if the liver cell line falls off and enters the body, it may cause tumors, and a cell that directly contacts blood can produce severe rejection and cause the cell to lose function