23-Hydroxyl betulinic acid kind derivant and preparing process, preparation and use thereof

A technology of betulinic acid and derivatives, applied in the field of natural medicine and medicinal chemistry

Inactive Publication Date: 2006-11-15
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, in the field of anti-tumor, most researchers believe that betulinic acid and its derivatives can achieve...

Method used

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  • 23-Hydroxyl betulinic acid kind derivant and preparing process, preparation and use thereof
  • 23-Hydroxyl betulinic acid kind derivant and preparing process, preparation and use thereof
  • 23-Hydroxyl betulinic acid kind derivant and preparing process, preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0193] 3,23-O-Diacetyl betulinic acid (X3)

[0194] Dissolve 23-hydroxybetulinic acid (0.12g, 0.25mmol) in 5ml of pyridine in a 10ml eggplant-shaped flask, add acetic anhydride (0.15ml, 1.5mmol) dropwise, and stir overnight at room temperature. Dilute with 10ml of ethyl acetate, wash with 9% hydrochloric acid to pH 4-5, wash with saturated brine (5ml×3), dry over anhydrous magnesium sulfate, filter, and recrystallize from ethyl acetate to obtain 0.136g of light yellow powdery solid. The rate is 98%.

[0195] ESI-MS: 579[M+Na] +

[0196] 1 HNMR (300Hz, CDCl 3 ), δ (ppm): 0.80 (s, 3H, CH 3 ), 0.88 (s, 3H, CH 3 ), 0.93 (s, 3H, CH 3 ), 0.97 (s, 3H, CH 3 ), 1.69 (s, 3H, CH 3 ), 2.01 (s, 3H, CH 3 ), 2.06(s, 3H, CH 3 ), 3.00 (m, 1H, C 19 -H), 3.68~3.70(d, 1H, 23-H), 3.83~3.85(d, 1H, 23-H), 4.61(S, 1H, 29-H), 4.74(s, 1H, 29-H ), 4.75~4.78(m, 1H, 3-H)

Embodiment 2

[0198] 3,23-O-Diacetyl-28-betulinoyl chloride (BYA-2)

[0199] Dissolve 3,23-O-diacetyl-28-acid-betulinic acid (0.1g, 0.18mmol) in 5ml of refined and dried dichloromethane in a 10ml eggplant-shaped bottle, add 0.1ml of oxalyl chloride dropwise, and stir at room temperature for 4h , the dichloromethane was distilled off. Refined and dried dichloromethane (5ml×3) was added, dissolved and evaporated. Finally, 0.1 g of a light yellow glassy solid was obtained.

Embodiment 3

[0201] 3,23-Diacetyl-28-methyl betulinate (SZ1)

[0202] Dissolve BYA-2 (0.1 g, 0.17 mmol) prepared by the method in Example 2 in a 10 ml eggplant-shaped flask with 5 ml of refined and dried dichloromethane, add 5 ml of methanol dropwise, and stir at room temperature for 6 h. Wash with saturated brine (5ml×3), dry over anhydrous sodium sulfate, filter, and concentrate the filtrate to make sand. Column chromatography [petroleum ether (60°C-90°C): ethyl acetate = 12:1 (v:v)] gave 0.092 g of a white powdery solid, with a total yield of 90.0%, and mp 110-112°C.

[0203] ESI-MS: 593[M+Na] +

[0204] 1 HNMR (300Hz, CDCl 3 ), δ (ppm): 0.78 (s, 3H, CH 3 ), 0.83 (s, 3H, CH 3 ), 0.87 (s, 3H, CH 3 ), 0.89 (s, 3H, CH 3 ), 1.68 (s, 3H, CH 3 ), 1.99 (s, 3H, CH 3 ), 2.04(s, 3H, CH 3 ), 2.94~3.01 (m, 1H, C 19 -H), 3.64(s, 3H, CH 3 -O), 3.59~3.68(d, 1H, 23-H), 3.79~3.83(d, 1H, 23-H), 4.57(s, 1H, 29-H), 4.71(s, 1H, 29-H ), 4.71~4.77(m, 1H, 3-H)

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Abstract

A kind of modified 23-hydroxy betulic acid derivatives, their preparing process, the medicinal composition containing them and their application in preventing and treating tumor are disclosed.

Description

technical field [0001] The present invention relates to the field of natural medicine and medicinal chemistry, in particular to a class of modified 23-hydroxy betulinic acid derivatives. The invention also discloses the preparation method of these 23-hydroxy betulinic acid derivatives, The pharmaceutical composition of the compound and the use of such 23-hydroxy betulinic acid derivatives for anti-tumor. Background technique [0002] 23-Hydroxybetulinic acid is a compound similar to betulinic acid in structure. 23-Hydroxybetulinic acid is a pentacyclic triterpenoid compound extracted from the Chinese herbal medicine Pulsatilla chinensis Regel. Belonging to the genus Pulsatilla in the family Ranunculaceae, it has the effects of clearing heat and detoxifying, cooling blood and stopping dysentery, drying dampness and killing insects. Clinically, it is mainly used to treat bacillary dysentery, amoebic dysentery, and gynecological vaginitis. Modern research has found that Psyll...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/56A61P35/00
Inventor 吴晓明徐进宜毕毅周金培张陆勇袁貹涛李丹徐萍
Owner CHINA PHARM UNIV
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