Preparation process of human recombined parathyroid hormone 1 84

A parathyroid hormone, -PTH84 technology, applied in the direction of parathyroid hormone, botany equipment and methods, microbial-based methods, etc., can solve the problems of low yield, difficult to scale up production, etc., to achieve high yield, excellent effect, The effect of omitting the renaturation step
CN1861790AInactive Publication Date: 2006-11-15EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2006-11-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparing method of the recombination human parathyroid hormone 1-84 by the gene engineering technology. It uses the codon for E.Coli and synthesizes the encoding sequence of the rhPTH1-84 and constructs the carrier and engineering bacteria. The invention is to express the soluble fusion protein contain the whole sequence of the hPTh 1-84 in the E.Coli cytoplasm, then the protein is purified by the chelate chromatography and cut by the enterokinase, next to purify by the ionic exchange and the gel chromatography to get the rhPTH 1-84 which the purity is more than 80%, the yield can reach 300mg / L.
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Description

technical field

[0001] The invention relates to the preparation of the active protein of parathyroid hormone 1-84, in particular to the preparation of recombinant human parathyroid hormone 1-84 by using genetic engineering technology. Background technique

[0002] Human parathyroid hormone (human parathyroid hormone, hPTH) is a linear peptide hormone secreted by the principal cells of the human parathyroid gland. The mature peptide is 84 amino acids in length and is an important hormone in the body that regulates calcium balance and bone remodeling. , can be used as a safe and effective medicine for the treatment of osteoporosis research and development. The current drugs for the treatment of osteoporosis are mainly osteoclast inhibitors, which only act on osteoclasts, can slow down or inhibit the activity of osteoclasts, and inhibit bone resorption, but have a poor effect on bone formation and cannot stimulate osteogenesis The cells produce strong new bone, and cannot rebu...

Claims

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