Process for producing hydroxyacetic acid by microbiological method

A glycolic acid and microbial method technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of polluted environment, complex process, low conversion rate, etc., and achieve high product quality, low energy consumption, The effect of low production cost

Inactive Publication Date: 2006-12-20
SHANDONG POLYMER BIO CHEM CO LTD
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Problems solved by technology

The traditional method of producing glycolic acid in the world is to hydrolyze hydroxyacetonitrile under the condition of a chemical cata...

Method used

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  • Process for producing hydroxyacetic acid by microbiological method

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Embodiment Construction

[0013] With reference to accompanying drawing, its detailed technical scheme comprises as follows:

[0014] (1) Screening of enzyme-producing cell strains

[0015] The nitrile hydratase with strong acid production ability is screened from the strains of Corynebacterium propionici, and then the strains with high enzyme production are screened for cultivation and amplification.

[0016] (2) Preparation of enzyme-producing cells

[0017] Add an appropriate amount of seed medium (10%-50%) into the triangular flask, insert the strains (about 5%) after sterilization, and culture at 20°C-40°C in a rotary shaker at 100-600rpm for 50-120°C. Hours, bottled for later use. Add an appropriate amount of seed culture medium (10%-50%) in the seed tank, insert the above-mentioned bacterial strains for bottling after sterilization, and at a temperature of 20°C-40°C, the ventilation rate is 1: 0.2-1 : 1 (V.V.M); Stirring speed: 50-400rpm, tank pressure 0.04-0.06, ferment for 50-120 hours; the...

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Abstract

The invention discloses a glycolic acid producing method through microbe, which comprises the following steps: (1) culturing ethylformic corynebacterium to produce nitrile hydration enzyme; (2) utilizing nitrile hydration enzyme as catalyst to produce low-density amide thioglycollic acid solution through hydroxyl acetonitrile hydration reaction; (3) evaporating low-density amide thioglycollic acid solution through drawing stickle to remove amide through ion exchange column to prepare high-density thioglycollic acid solution; (4) crystallizing and drying high-density thioglycollic acid solution at low temperature to prepare high-purity glycolic acid.

Description

1. Technical field: [0001] The invention relates to a microbial method for producing glycolic acid by using biological enzymes as catalysts to produce glycolic acid through the hydrolysis reaction of glycolic acid. 2. Background technology: [0002] Glycolic acid is a fine organic chemical raw material produced in large tonnage in major industrialized countries and has a wide range of uses. There is no manufacturer of glycolic acid on an industrial scale in my country, and the market needs glycolic acid and related products completely dependent on imports. The traditional method of producing glycolic acid in the world is to hydrolyze hydroxyacetonitrile under the condition of a chemical catalyst, which has the disadvantages of complex process, low conversion rate, high energy consumption, environmental pollution, and high cost. 3. Contents of the invention: [0003] The purpose of the present invention is exactly to aim at the above-mentioned defect that prior art exists,...

Claims

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Application Information

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IPC IPC(8): C12P7/42C12R1/15
Inventor 夏春良吴时军刘皓周卫东孙中华付纲胡奎玲王曰鹏
Owner SHANDONG POLYMER BIO CHEM CO LTD
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