Bupleurum root extract, its preparing method and its use

A Bupleurum extract and extract technology, applied in the field of traditional Chinese medicine, can solve the problems of low content of active ingredients, unfavorable drug efficacy, large toxic and side effects, etc.

Inactive Publication Date: 2007-01-24
郭爱华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the confusion of the varieties of medicinal materials used, the inconsistency of medicinal parts, and the complexity of its chemical composition, the content of active ingredients varies greatly, resulting in unstable quality of Bupleurum and its preparations, and there are certain gaps in its curative effect or use. Problem: Oral preparations such as tablets, granules, granules, etc. have the disadvantages of large doses and ineffective effects. Each time you need to take 4-6 tablets or 10-20g, which brings inconvenience to patients; while injections have serious side effects , leading to adverse reactions and other defects
At present, in the existing saikosaponin products, the content of active ingredients is low and the content of impurities is high, which is not conducive to the development of drug efficacy and related dosage forms. Therefore, the development of high-purity saikosaponin products is particularly important

Method used

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  • Bupleurum root extract, its preparing method and its use
  • Bupleurum root extract, its preparing method and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] Wash and dry Bupleurum Radix, crush it to 10 mesh, add 8 times the weight of Bupleurum Radix with 70% ethanol, reflux extraction in 80°C water bath for 3 times, each time for 1 hour, combine the three extracts, concentrate under reduced pressure at 45°C, the concentrated solution The amount is controlled to be 3 times the amount of Bupleurum bupleuri, put the concentrated solution on a D101 macroporous resin column, let it stand for adsorption for 2 hours, then wash the column with distilled water 10 times the volume of the resin column to remove sugar, and then use 1 times the volume of the resin column to remove sugar. Wash the column with 50% ethanol to remove impurities; elute with 70% ethanol that is 5 times the volume of the resin column, collect the eluate, put it on a D280 decolorization column, and concentrate it under reduced pressure at 45°C after decolorization. The specific gravity of the concentrate is controlled At 1.05, the Bupleurum bupleuri extract was ...

Embodiment 2

[0123] After washing and drying Bupleuri, crush it to 20 mesh, add 60% ethanol 10 times the weight of Bupleurum, and reflux extract twice in a water bath at 85°C for 2 hours each time, combine the extracts, concentrate under reduced pressure at 40°C, and concentrate Controlled to twice the amount of Bupleurum bupleuri, put the concentrated solution on a D101 macroporous resin column, let it stand for adsorption for 3 hours, then wash the column with distilled water 8 times the volume of the resin column to remove sugar, and then use 2 times the volume of the resin column to remove sugar. Wash the column with 40% ethanol by volume to remove impurities; elute with 60% ethanol of 6 times the resin column loading volume, collect the eluate, put it on a D280 decolorization column, and concentrate it under reduced pressure at 40°C after decolorization. The specific gravity of the concentrated solution is controlled at 1.20, dried to obtain the Bupleurum radix extract, after testing, ...

Embodiment 3

[0125] After washing and drying Bupleurum, crush it to 20 mesh, add 80% ethanol with 6 times the weight of Bupleurum, reflux extraction in a 75°C water bath for 3 hours, concentrate the extract under reduced pressure at 50°C, and control the amount of the concentrated solution to the amount of Bupleurum herbal medicine 4 times of the concentration, put the concentrated solution on the D101 macroporous resin column, let it stand for adsorption for 2 hours, then wash the column with distilled water 12 times the volume of the resin column to remove sugar, and then wash it with 1.5 times the volume of the resin column with 60% ethanol Remove impurities from the column; elute with 80% ethanol 4 times the volume of the resin column, collect the eluate, put it on a D280 decolorization column, and concentrate under reduced pressure at 50°C after decolorization. The specific gravity of the concentrated solution is controlled at 1.10, and dried to obtain chai The extract of Herba chinens...

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Abstract

The present invention is a kind of bupleurum root extract and its extracting process and medicinal use, and belongs to the field of Chinese medicine technology. The bupleurum root extract is obtained through extracting bupleurum root with alcohol solution, and contains bupleurum saponin-a in 20-30 wt% and bupleurum saponin-d in 10-20 wt%. The preparation process includes crushing bupleurum root, extraction, concentration, column chromatography, eliminating saccharide, depurating, eluting, decolorizing, concentrating, drying and other steps. The extract has high effective component content, high purity, and excellent medicinal application for resisting inflammation, resisting virus, resisting tumor, regulating incretion, etc.

Description

technical field [0001] The invention relates to a Bupleurum bupleuri extract, its preparation method and application, and belongs to the technical field of traditional Chinese medicine. Background technique [0002] Bupleurum is a plant of the genus Bupleurum in the family Umbelliferae, and its roots are used as medicine. There are more than 120 species of plants in this genus, 42 species and 17 varieties in my country, and more than 10 species are widely used among the people. Only Bupleu rumscorzonerifolium Willd and B.chinense DC are recorded in the Pharmacopoeia of the People's Republic of China. It is bitter in taste and cool in nature, and enters the liver and gallbladder meridians. It has the functions of reconciling the exterior and interior, soothing the liver, and promoting yang. Indications for alternating cold and heat, full chest and rib pain, bitter mouth and deafness, headache and dizziness, malaria, diarrhea, prolapse of the anus, irregular menstruation, u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/233A61K9/00A61P11/00A61P29/00A61P31/16A61P13/12A61P1/16A61P35/00A61K125/00
Inventor 郭爱华
Owner 郭爱华
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