Unlock instant, AI-driven research and patent intelligence for your innovation.

Amylose content control gene DU1 of rice endosperm and application thereof

A technology of amylose content and endosperm, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as unseen cloned genes

Inactive Publication Date: 2007-02-07
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reports of cloning the gene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Amylose content control gene DU1 of rice endosperm and application thereof
  • Amylose content control gene DU1 of rice endosperm and application thereof
  • Amylose content control gene DU1 of rice endosperm and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Cloning of rice endosperm amylose content control gene DU1

[0029] 1. Rice material

[0030] Rice (Oryza sativa ssp.) endosperm low-amylose mutant du1 (obtained by the China Rice Research Institute using EMS (ethyl methanesulfonate) mutagenesis) [6，7] and conventional rice variety Minghui 63 (purchased from China Rice Research Institute).

[0031] 2. Analyze and target groups

[0032] The homozygous indica variety Minghui 63 was crossed with the japonica mutant du1, and F 1 A total of 7,150 F 2 Individuals, and 1,618 individuals were selected as the positioning group. Take about 2 grams of young leaves from each plant at the seedling stage to extract DNA.

[0033] 3. Locate the DU1 gene by SSR (Simple Sequence Repeat), STS (Sequence-tagged Sites), and CAPS (Cleaved amplified polymorphic sequence) markers

[0034] Using the improved CTAB (Cetyltrimethyl Ammonium Bromide) method [9] Genomic DNA for gene mapping was extracted from rice leaves. About 100m...

Embodiment 2

[0044] Example 2 Functional complementation and transgenic research of rice endosperm amylose content control gene SU1

[0045] Primers were designed according to the sequence of the indica rice Minghui 63 DU1 gene, and the primers DU1F, DU1R, DU2F, DU2R, DU3F and DU3R (see Table 1 for the sequence) were used to divide into three sections of high-fidelity PCR (see Table 1 for the sequence, pre-denaturation at 94°C for 5 min, 94 1 min at ℃, 1 min at 60 °C, 1 min at 72 °C, 35 cycles, 72 °C extension for 10 mins and sequencing with ABI3730 DNA analyzers (ABI Company, USA), and select clones with completely correct sequences using the shared Sal I and Xba I sites They were ligated into a 8.3kb fragment, comprising 3,055 bases upstream of the start codon ATG and the full-length sequence of 2,115 bases after the stop codon TAG, and cloned into the binary vector pCAMBIA1300 (purchased from CAMIA Company, Australia ), the plasmid pCAMBIDU1 ( Figure 5 ). The plasmid was transferred ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

the invention discloses a gene DU1, which controls straight-chain starch content of rice endosperm in the rice beauty-water 11, wherein the gene ribonucleotide sequence is selected from (1) the ribonucleotide sequence of amino acid sequence of coded SEQ ID NO: 2; (2) the ribonucleotide sequence to control plant organ starch composition and rice endosperm straight-chain starch content simultaneously. the invention also provides protein to control straight-chain starch content of rice endosperm and plant expressive carrier of gene as well as plant cultivating method, which comprises the following steps: converting the gene of expressive carrier into plant cell; cultivating the converted plant cell into plant.

Description

technical field [0001] The present invention relates to the field of plant genetic engineering, more specifically, the present invention relates to rice endosperm amylose content control gene DU1, the protein encoded by the gene and its functional analogue, the nucleotide sequence encoding it, and the nucleotide sequence containing the nucleotide A vector and a host cell containing the vector; in addition, the present invention also relates to a method for controlling the amylose content of the plant endosperm and a breeding method for improving the amylose content of the plant endosperm. Background technique [0002] Rice endosperm starch is usually composed of amylose and amylopectin, with a content of about 90%. [1] . Amylose refers to a linear long-chain molecule with a length of about 1000 glucose residues formed by α-1,4 glycosidic bonds between glucose units, while amylopectin is formed on this basis with α-1,6 Glycosidic bonds derive small chain-like molecules with...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H4/00
Inventor 李家洋钱前曾大力田志喜周奕华
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com