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Polysaccharide from phellinus igniarius 4.6302 strain and preparation method thereof

A Phellinus and mycelia technology, which is applied in the field of microbial active ingredients and its preparation, can solve the problems of differences in medicinal effects of wild Phellinus fungus, huge investment in Phellinus mycelium, and long Phellinus cycle, which is beneficial to quality control , short cycle, stable curative effect

Active Publication Date: 2010-09-01
安徽省丹皇本草科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the wild resources of Phellinus japonica have been exploited extinctly, and the price is expensive, and the supply exceeds the demand
Due to the influence of many factors such as climate and environment, the success rate of artificial cultivation of Phellinus japonica for a long period is low
[0005] Phellinus mycelium can be produced by liquid fermentation method, but this method requires a huge investment to produce Phellinus mycelium, and it is difficult to realize it in a short time
[0006] The medicinal effects of wild Phellinus fungus vary significantly due to differences in species, hosts, and origins

Method used

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  • Polysaccharide from phellinus igniarius 4.6302 strain and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] (1) Preparation of solid medium

[0041] Table 1 Unit: Gram

[0042]

[0043] Weigh each component according to the formula in Table 1 and mix evenly, adjust the pH to 6-8 with NaOH and HCl or organic acid or alkali, and sterilize for later use.

[0044] (2), Phellinus strain 4.6302 solid-state fermentation culture

[0045] 1. Cultivation of Phellinus 4.6302 strain test tube strain slant parent species

[0046] Medium: PDA comprehensive medium

[0047] Culture conditions: 6-9 days under dark light at 23-29°C, with natural pH.

[0048] 2. Shake flask seed culture

[0049] Use an inoculation loop to pick out 2 to 3 pieces of bacteria that have been activated in step (1) and inoculate them into a 500ml triangular flask liquid seed medium with a size of 1cm × 1cm. Plug it and wrap the outer layer with kraft paper.

[0050] Medium components (weight): 20-30g sucrose, 3-4g yeast extract, 3-4g peptone, MgSO 4 ·7H 2 O 0.5g, KH 2 PO 4 0.1g, water 1000ml.

[0051] C...

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Abstract

the invention discloses a morin 4.6302 strain fungal polysaccharide, which is cultivated by 60-80% rice bran, 8-12% corn powder, 8-12% soy powder, 0.8-2.2% glucose, 0.8-2.2% beef grease, 0.04-0.06% MnSO4 and 80-120% water at 22-30 deg.c for 10-20 days.

Description

1. Technical field [0001] The invention relates to a microbial active ingredient and a preparation method thereof, in particular to a medicinal fungal active ingredient and a preparation method thereof, specifically a Phellinus 4.6302 strain mycoplasma polysaccharide and a preparation method thereof. 2. Background technology [0002] Phellinus Basidiomycota, Phytomycetes, Phyllomycetes, Polyporaceae medicinal fungi. Morus On the plant, and the fruiting body is yellowish brown, so it is named. Phellinus has extremely high medicinal value, and has significant effects in enhancing human immunity, anti-hepatic fibrosis, anti-lipid peroxidation, anti-inflammation, etc., especially in anti-tumor. The most effective fungus for cancer. [0003] Phellinus polysaccharides can be applied to the biological effects of anti-tumor, anti-mutation, anti-oxidation, anti-hepatic fibrosis, anti-aging and inhibiting the growth of cells such as HepG-2, breast cancer cell lines MCF-7 and Hel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00C12R1/645
Inventor 沈业寿史新强郑立军李宜明赵根海郑媛
Owner 安徽省丹皇本草科技有限公司