Stabilized phosphatase formulations

A stabilizer and phosphatase technology, applied in the direction of enzyme stabilization, enzymes, hydrolytic enzymes, etc., can solve problems such as insufficient internal resistance

Inactive Publication Date: 2007-02-07
BASF AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most industrial phosphatases currently available in feed and / or food applications have insufficient intrinsic resistance to heat inactivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment V1

[0209] Example V1 (comparison)

[0210] The cornstarch-based enzyme granules were prepared by kneading, extrusion, spheronization and drying.

[0211] In the first step, cornstarch was charged into a laboratory mixer (Loedige Typ M5 RMK) and agitated slowly. The ultrafiltrate containing phytase, ZnSO 4 ×6H 2 A mixture of O, PVA (polyvinyl alcohol) and additional water was poured into a beaker and dissolved / dispersed. Pour this mixture over the cornstarch in a Loedige mixer.

[0212] Mix and knead 66% (w / w) corn starch, 20% (w / w) ultrafiltrate containing phytase, 1% (w / w) PVA, 0.3% (w / w) ZnSO 4 ×6H 2 O and 12.7% (w / w) additional water to obtain an enzyme-containing dough.

[0213] The second step was to extrude the enzyme-containing dough with a Fuji Paudal laboratory extruder (model DG-L1) to obtain a wet extrudate, which was then spheronized in a laboratory spheronizer to obtain an average diameter of 500-800 μm round particles.

[0214] Reality

Embodiment 1

[0215] Example 1: Casein as stabilizer

[0216] First step Corn starch and casein (available from Merck, Darmstadt, CAS No. 900-71-9) were charged into a laboratory mixer (Loedige Typ M5 RMK) and agitated slowly. The ultrafiltrate containing phytase, ZnSO 4 ×6H 2 A mixture of O and additional water was poured into a beaker and dissolved / dispersed. Pour this mixture over the cornstarch and casein in a Loedige mixer. A phytase-containing dough is thus obtained by mixing and kneading the ingredients in a Loedige mixer at 100-250 rpm.

[0217] Reality

Embodiment 2

[0218] Example 2: Casein as stabilizer

[0219] Reality

Example 2

corn

starch

Ultrafiltration

liquid

cheese eggs

White

ZnSO 4

×6H 2 O

water

pregnancy

dough

47.0％

20.0％

20.0％

0.3％

12.7％

grain

grain

59.6％

8.6％

25.4％

0.4％

6.0％

[0220] Granulation stability comparison

[0221] Granulation tests were carried out on the phytase granules of the invention and their granulation stability was compared with granules produced according to the standard formulation (=Example V1). The pelleting test consisted of mixing different enzyme pellets with standard broiler food at a level of 1 g / kg feed. The enzymatic feed was treated by injecting steam in a thermostat followed by pelleting in a tablet press to obtain feed tablets, followed by cooling. The pellet temperature measured directly after the tablet pr...

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Abstract

The invention relates to a stabilized solid or liquid enzyme formulation comprising at least one phosphatase and at least one stabilizing agent selected from the group consisting of agar, algin, carrageenan, furcelleran, ghatti gum, tragacanth gum, gum karya, guaran, locust bean gum (carob bean gum), tamarind seed gum, arabinogalactan, xanthan (gum), at least one animal protein and mixtures thereof, with the proviso that if gelatine is used in granules as solid formulations as the only stabilizing agent, the granules are subsequently coated. The invention also relates to processes for producing these formulations, especially to solid formulations (e.g. granules) and their use in animal and / or human nutrition.

Description

technical field [0001] The present invention relates to solid or liquid phosphatase formulations with improved stability, preferably thermal stability, obtained by adding stabilizers. [0002] The present invention relates to the formulation of phosphatases, preferably phytases, into granular and / or liquid formulations containing carbohydrates, such as starch, and to methods of making such enzyme-containing granules and liquid formulations. These (edible) granules and liquid formulations can be used in animal feed and / or human nutrition. Enzyme-containing granules and liquid formulations exhibit improved storage and processing stability. Background technique [0003] The use of phosphatases in human nutrition or animal (eg livestock) feed has become almost common practice. These enzymes are typically produced by culturing microorganisms in large scale fermenters operated by industrial enzyme producers. The resulting "fermentation broth" is typically subjected to a series ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/00C12N9/14A23K1/165A23L1/00A23L29/00A23L29/20C12N9/96C12N9/98
CPCC12N9/98A23K1/001A23K1/002A23K1/003A23K1/004A23K1/1653A23L1/0029A23L1/034C12N9/96A23K40/00A23K40/10A23K40/20A23K40/30A23K20/189A23P10/30A23L29/06A23K40/25A23L29/00
Inventor A·哈比希J·布劳恩
Owner BASF AG
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