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Organic group functionized mesoporous molecular sieve enzyme immobilized carrier, and its preparing method

A technology of immobilized carrier and mesoporous molecular sieve, which is applied in the direction of immobilization on or in the inorganic carrier, can solve the problem of late start of research on immobilized carrier materials, and achieve the effect of mild immobilization reaction conditions and simple operation process

Inactive Publication Date: 2007-03-28
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country has made some achievements in the research of immobilized enzyme technology, but the research on immobilized carrier materials started relatively late, so far it still needs to import a large amount of immobilized penicillin acylase and carriers to meet the needs of the semi-synthetic antibiotic industry. The reason is that there is no commercial immobilization carrier in China

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  • Organic group functionized mesoporous molecular sieve enzyme immobilized carrier, and its preparing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of epoxidized mesoporous carrier by grafting method.

[0033] The mass ratio of the raw materials is: toluene: polyoxyethylene ether-polyoxypropylene ether-polyoxyethylene ether: ethyl orthosilicate: hydrochloric acid=1:1:1.5:35. Among them, toluene is a pore expander. The operation is as follows: Dissolve polyoxyethylene ether-polyoxypropylene ether-polyoxyethylene ether in an HCl solution at about 30°C. The concentration of the HCl solution is 1.0 mol / L. Under stirring, slowly add toluene and ethyl orthosilicate in sequence After the obtained sol was continuously stirred for 24 hours, it was transferred to a crystallization kettle and allowed to stand for crystallization at 110°C for 24 hours. The obtained colloid is filtered, washed, dried at 90 DEG C, and calcined at 500 DEG C for at least 15 hours to obtain the original powder of mesoporous molecular sieve. The raw powder of mesoporous molecular sieve is vacuum degassed and put into a three-necked flask, an...

Embodiment 2

[0037] Preparation of epoxidized mesoporous carrier by grafting method.

[0038] The mass ratio of the raw materials is: trimethylbenzene: polyoxyethylene ether-polyoxypropylene ether-polyoxyethylene ether: ethyl orthosilicate: hydrochloric acid = 0.1:1:4.5:70. Among them, trimethylbenzene is a hole expander. The operation is as follows: Dissolve polyoxyethylene ether-polyoxypropylene ether-polyoxyethylene ether in a 1.8mol / L HCl solution at 40°C, slowly add ethyl orthosilicate with stirring, and the resulting sol will continue to be stirred for 48 hours, then turn Put it into the crystallization kettle and let it stand for crystallization at 120°C for 30h. The obtained colloid is filtered, washed, dried at 120°C, and calcined at 650°C for at least 8 hours to obtain the original powder of mesoporous molecular sieve. The raw powder of mesoporous molecular sieve is vacuum degassed and put into a three-necked flask, and 20% of its mass is added with 3-oxyglycidyl-propyl-trimethoxysil...

Embodiment 3

[0041] Preparation of aminated mesoporous carrier by grafting method.

[0042] The mass ratio of the reagents is: trimethylbenzene: polyoxyethylene ether-polyoxypropylene ether-polyoxyethylene ether: ethyl orthosilicate: hydrochloric acid = 0.2:1:2.5:45. Among them, trimethylbenzene is a hole expander. The operation is as follows: Dissolve polyoxyethylene ether-polyoxypropylene ether-polyoxyethylene ether in a 1.8mol / L HCl solution at 35℃, add trimethylbenzene and ethyl orthosilicate with stirring, and the resulting sol is continuously stirred for 24 hours. , Transferred to the crystallization kettle and allowed to stand for crystallization at 100°C for 24h. The obtained colloid is filtered, washed, and finally extracted with ethanol in a Soxhlet extractor to remove polyoxyethylene ether-polyoxypropylene ether-polyoxyethylene ether to obtain mesoporous molecular sieve raw powder. The raw powder of mesoporous molecular sieve is dispersed in toluene, and the mass ratio of γ-aminopro...

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Abstract

The invention relates to organic group functionalized silica mesoporous molecular sieve enzyme immobilized carrier and the preparing method. The carrier includes the silica mesoporous molecular sieve on which there is organic group functional group. It includes silica mesoporous molecular sieve composing and organic group functional group transplanting. The average aperture of the carrier is 5-20nm. Specific surface area is 40-600m2 / g. The carrier immobilized enzyme apparent activity is 1000-2000IU / g which can be over 90% after 10 times using. The produced functionalized silica mesoporous molecular sieve can immobilize biological enzyme molecular under moderate condition by covalent union to make the immobilized penicillin acylase has high catalytic activity and stability in use.

Description

Technical field [0001] The invention relates to an immobilized enzyme biotechnology, in particular to an organic group functionalized mesoporous molecular sieve enzyme immobilized carrier and a preparation method thereof. Background technique [0002] As a biocatalyst, enzyme has a high degree of specificity—optical, stereo or group selectivity. It can catalyze efficiently at neutral pH and room temperature, and its catalytic ability is much higher than that of chemical catalysts. Although enzymes can catalyze many chemical reactions in organisms, they have serious defects when used as industrial catalysts. They are very sensitive to the environment in which they are located. They are unstable in acids, alkalis, high temperatures, and organic solvents, and are prone to lose their catalytic activity. The free enzyme is directly used in the catalytic reaction process, and the enzyme solution not only cannot be recovered, it also causes difficulties in product separation and purific...

Claims

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Application Information

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IPC IPC(8): C12N11/14
Inventor 薛屏徐立冬刘万毅刘成敏
Owner NINGXIA UNIVERSITY
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