Application of a Functionalized Ionic Liquid Modified Mesoporous Molecular Sieve in Enzyme Immobilization

A technology of mesoporous molecular sieve and ionic liquid, which is applied in the direction of immobilization on/in the organic carrier, can solve the problems of mass transfer limitation, large amount of ionic liquid, high viscosity, etc., and achieve mild immobilization reaction conditions, activity and stability The effect of high reliability and simple operation process

Inactive Publication Date: 2012-02-01
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, at present, functionalized ionic liquids are basically used as reaction media in enzyme-catalyzed reactions, and the amount of use is large and the cost is high. In addition, functionalized ionic liquids are difficult to separate from products in some enzyme-catalyzed reactions and have high viscosity. problems such as limiting mass transfer
In recent years, patents such as JP2009207492, JP2009203454, WO2008090156, and CN101225427 have used the "green" solvent and "designable solvent" properties of functionalized ionic liquids to simplify the product separation process and improve the stability or activity of the enzyme, but the same Encountered the problem that the amount of ionic liquid was too large, which was not suitable for industrial production, and the stability was improved, but it was still not as good as the stability of immobilized enzymes

Method used

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  • Application of a Functionalized Ionic Liquid Modified Mesoporous Molecular Sieve in Enzyme Immobilization
  • Application of a Functionalized Ionic Liquid Modified Mesoporous Molecular Sieve in Enzyme Immobilization
  • Application of a Functionalized Ionic Liquid Modified Mesoporous Molecular Sieve in Enzyme Immobilization

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Embodiment 1

[0037] 1) Synthesis of mesoporous molecular sieve SBA-15: According to the description of Zhao Dongyuan et al. (Triblock Copolymer Syntheses of Mesoporous Silica with Periodic 50 to 300 Angstrom Pores. Science, 1998, 279: 548-553), the original powder of SBA-15 molecular sieve was prepared , with a pore size of 10nm and a specific surface area of ​​350m 2 / g.

[0038] 2) Synthesis of functionalized ionic liquid: 1-methylimidazole and chloropropyltrimethoxysilane were refluxed at 120°C for 40 hours, cooled to room temperature, and the obtained product was stirred with KPF6 in acetone at room temperature for three days to obtain Functionalized ionic liquid crude liquid, the ionic liquid crude liquid is filtered, washed, separated, and acetone and water are distilled off under reduced pressure to obtain a functionalized ionic liquid, wherein 1-methylimidazole, chloropropyltrimethoxysilane and KPF 6 The molar ratio is 1:1:1.

[0039] 3) Preparation of functionalized ionic liqui...

Embodiment 2

[0053] 1) Synthesis of mesoporous molecular sieve MCM-48: According to the description of Mobil Company in (Ordered mesoporous molecular sievessynthesized by a liquid-crystal template mechanism. Nature, 1992.359 (6397): 710-712), the original MCM-41 molecular sieve was prepared powder, the pore size is 5nm, and the specific surface area is 1500m 2 / g.

[0054] 2) Synthesis of functionalized ionic liquid: 1-butylimidazole and chloropropyltrimethoxysilane were refluxed at 120°C for 40 hours (with a drying tube), cooled to room temperature to obtain the product. The obtained product was mixed with KPF 6 Stir in acetone at room temperature for three days to obtain an ionic liquid. The obtained product is filtered, washed, separated, and acetone and water are distilled off under reduced pressure to obtain an ionic liquid, in which 1-butylimidazole, chloropropyltrimethoxysilane and KPF 6 The molar ratio is 1:1:1.

[0055] 3) Preparation of functionalized ionic liquid modified meso...

Embodiment 3

[0058] 5) 1) Synthesis of mesoporous molecular sieve MCM-48: According to the description of Mobil Corporation in (Ordered mesoporous molecular sievessynthesized by a liquid-crystal template mechanism. Nature, 1992.359(6397): 710-712), MCM-48 was prepared Molecular sieve raw powder, the pore size is 8nm, the specific surface area is 1400m 2 / g.

[0059] 2) Synthesis of functionalized ionic liquid: 1-ethylimidazole and chloropropyltrimethoxysilane were reacted under reflux at 120°C for 40 hours (with a drying tube), cooled to room temperature to obtain the product. Combine the obtained product with KPF 6 Stir in acetone at room temperature for three days to obtain an ionic liquid. The obtained product is filtered, washed, separated, and acetone and water are distilled off under reduced pressure to obtain an ionic liquid. Among them, 1-ethylimidazole, chloropropyltrimethoxysilane and KPF 6 The molar ratio is 1:1:1.

[0060] 3) Preparation of functionalized ionic liquid modifi...

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Abstract

The invention belongs to the field of immobilized enzyme carriers and discloses the application of a functionalized ionic liquid modified mesoporous molecular sieve in enzyme immobilization. The surface of the mesoporous molecular sieve can interact with the organic functional group from the functionalized ionic liquid that can be combined with the amino acid residue of the enzyme. The present invention utilizes the designability of ionic liquids to introduce functionalized ionic liquids with functional groups such as alkyl, amino, carboxyl, and hydroxyl groups on the surface of mesoporous materials, through which electrostatic and hydrogen bonds can occur with amino acid residues in enzyme molecules , hydrophobic bonds, etc., enhance the binding force between the carrier and the enzyme, change the microenvironment of the enzyme, and improve the catalytic performance of the immobilized enzyme. The present invention uses functional ionic liquids to modify mesoporous materials for the immobilization of enzymes, which not only proposes a new concept, but also enriches the surface modification methods of mesoporous materials, and also expands the use of ionic liquids and mesoporous materials in enzyme immobilization. applications in engineering.

Description

technical field [0001] The invention belongs to the field of catalyst carriers and relates to the application of a functionalized ionic liquid-modified mesoporous molecular sieve in enzyme immobilization. Background technique [0002] As a catalyst, enzymes have the characteristics of high efficiency, specificity, mildness, and environmental friendliness, and are widely used in food, medicine, and chemical industries. Among them, compared with free enzymes, immobilized enzymes have the advantages of good stability, easy separation from the reaction system, reusability, convenient transportation and storage, and conducive to continuous and automatic production, so they have a wider application space. As a part of the immobilized enzyme, the carrier material has a great influence on the performance of the immobilized enzyme by its structure and performance. For example, the specific surface area, pore size distribution, pore volume, topological structure, surface properties, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/04
Inventor 胡燚黄和邹彬唐苏苏刘维明
Owner NANJING TECH UNIV
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