Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Compound and its preparation method and its application in pharmacy

A technology of compounds and strains, which is applied in the field of compounds with antifungal effects, can solve the problems of no relevant reports on the active ingredients of antifungal substances, and achieve good antibacterial activity and antibacterial effect

Inactive Publication Date: 2007-04-25
HANGZHOU XIAYANG BIOENG
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some endophytic fungi in Euphorbia fischeriana Stued., but there is no relevant report on the endophytic fungi and their antibacterial active ingredients of these Euphorbia fischeriana Stued.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound and its preparation method and its application in pharmacy
  • Compound and its preparation method and its application in pharmacy
  • Compound and its preparation method and its application in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] 1. Bacterial strain: the fermentation strain adopts the strain with the preservation number of CCTCC NO: M206095.

[0081] 2. Culture medium:

[0082] Slant medium: 20% potato juice, add 20g glucose, 10g ammonium nitrate, 15g agar, 0.1g sodium chloride, natural pH;

[0083] Seed medium: 20% potato juice, adding 25g of glucose, 12g of ammonium nitrate, 0.1g of sodium chloride, natural pH;

[0084] Fermentation medium: 20 g of acetyl starch, 20 g of glucose, 15 g of corn steep liquor (40% solid content), 0.1 g of sodium chloride, dissolved in appropriate amount of tap water, adjusting pH to 6.8-7.0 with 10% NaOH solution, adding tap water to set Capacity up to 1000ml;

[0085] The medium was sterilized at 121°C for 30 min.

[0086] 3. Training method:

[0087] Slant culture: Dissolve and inoculate the cultured strain with the preservation number CCTCC NO: M206095 in aseptic water on the slant medium, culture it at 26°C for 7 days, and inoculate it with 30% sterile gly...

Embodiment 2

[0093] 1. Strain: the fermentation strain adopts the strain with the preservation number CCTCC NO: M206095;

[0094] 2. Culture medium:

[0095] Incline medium: glucose 120g, ammonium sulfate 20g, agar 15g, add tap water to 1000ml, natural pH;

[0096] Seed in medium: glucose 130g, ammonium sulfate 20g, add tap water to 1000ml, natural pH;

[0097] Fermentation medium: 100g of sucrose, 5g of corn steep liquor with a solid content of 40%, 5g of peptone, add tap water, adjust the pH to 6.8-7.0 with 10% NaOH solution, and set the volume to 1000ml;

[0098] The medium was sterilized at 121°C for 30 min.

[0099] 3. Training method:

[0100] Slant culture: Dissolve and inoculate the cultured strain with the preservation number CCTCC NO: M206095 stored at -70°C in aseptic water and streak on the slant medium, culture at 28°C for 5 days, and use 30% sterile glycerol The spores were washed and stored at -70°C for later use.

[0101] Preparation of seed solution: Inoculate the spo...

Embodiment 3

[0106] 1. Strain: the fermentation strain adopts the strain with the preservation number CCTCC NO: M206095;

[0107] 2. Culture medium:

[0108] Incline medium: molasses 40g, bean cake 80g, agar 15g, ferrous sulfate 0.05g, add distilled water to 1000ml, natural pH;

[0109] Seed medium: molasses 40g, bean cake 80g, ferrous sulfate 0.05g, add distilled water to 1000ml, natural pH;

[0110] Fermentation medium: molasses 60g, fish peptone 5g, bean cake 20g, ferrous sulfate 0.05g, add distilled water to 1000ml, natural pH;

[0111] The medium was sterilized at 121°C for 30 min.

[0112] 3. Training method:

[0113] Slant culture: Inoculate the slant culture medium with the preservation number CCTCC NO: M206095 strain frozen at -70°C in aseptic water, dilute and streak on the slant medium, culture at 28°C for 5 days, wash with 30% sterile glycerol The spores were stored at -70°C for later use.

[0114] Preparation of seed solution: Inoculate the spores of the bacterial strain ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
purityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a compound and preparing method and application in the drug, which is 2, 3-diamino-6-hydroxy-benzene carbonic acid-2-ethyl group-hexaester fermented by M 206095 strain with excellent antimycotic action to prepare antimycotic drug.

Description

technical field [0001] The invention relates to a compound with antifungal effect, and also relates to the preparation method and application of the compound in pharmacy. Background technique [0002] Chamaejasma is the root of Stellera chamaejasm eL. or Euphorbia fischeriana Stued. and Euphorbia ebracteolata Hayata. , The effect of dispelling stagnation and killing insects is a commonly used traditional Chinese medicine. Studies in recent years have found that chamaejasma extracts have strong inhibitory activity on tumor cells, in addition to antibacterial, antiviral and insecticidal effects, and are an ideal natural drug development variety. There are some endophytic fungi in Euphorbia fischeriana Stued. plants, but there is no relevant report on these endophytic fungi and their antibacterial active ingredients. Contents of the invention [0003] The object of the present invention is to provide a compound for the above problems, which has good antifungal effect and ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07C229/64C12N1/14C12P13/00A61K31/216A61P31/10C12R1/685
Inventor 夏国兴陈有为张宇楷王欣陈晓霞朱健李邦良
Owner HANGZHOU XIAYANG BIOENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com