Process for extracting free mRNA from pleural fluid and ascitic fluid

Abstract

The invention concerns a method for extracting free mRNA from pleural fluid and abdominal dropsy. In the invention injecting pleural fluid and abdominal dropsy into an anticoagulation tube with natrium citricum without RNase, centrifugating, imbibing the supernatant, centrifugating again, imbibing the supernatant, adding the supernatant into Trizol LS solution, shaking at room temperature, addingchloroform, standing at room temperature, centrifugating and imbiling water phase layer to a new tube, adding isopropyl alcohol for RNA precipitation, centrifugating, washing with 75% ethanol, air-drying, adding RNase-free water, and dissolving RNA anti-transcribing with the RNA as templates using conventional technology into cDNA for preservation. The invention dissolves the problem that RNA cannot be extracted form pleural fluid and abdominal dropsy in the past. The invention can provide early treatment for cancer and other diseases. The invention has the advantages of easy operation and high reproducibility.

Description

technical field [0001] The invention relates to a biotechnology extraction method, in particular to a method for extracting free mRNA from chest and ascites. Background technique [0002] Before the present invention, because pleural effusion and ascites were often malignant tumor metastasis or the first clinical manifestation, it was often done in medical practice to extract a certain volume of pleural and ascites for malignant tumor detection. However, due to the relatively low pathological detection rate of tumor cells and the need for a certain number of pleural effusion and ascites samples, patients with pleural and peritoneal effusions often need to be repeatedly sent for examination in clinical practice, so it is difficult to make a clear diagnosis in time or because there are not enough samples. Due to the large number of specimens, it is impossible to accurately diagnose and delay the best treatment opportunity; at the same time, in the detection of tumor-associated...

AI Technical Summary

Problems solved by technology

However, due to the relatively low pathological detection rate of tumor cells and the need for a certain number of pleural effusion and ascites samples, patients with pleural and peritoneal effusions often need to be repeatedly sent for examination in clinical practice, so it is difficult to make a clear diagnosis in time or because there are not enough samples. Due to the large number of specimens, it is impossible to accurately diagnose and delay the best time for treatment; at the same time, in the detection of tumor-associated antigens in pleural and ascites, the detection objects of traditional methods are limited to related proteins and cells in pleural effusion and ascites, and the sensitivity is low. Conducive to early diagnosis of disease and judgment of curative effect

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