Method for producing biological sensor

Abstract

The method includes steps: (1) stretching air core quartz glass rod, cooling the drawn down rod, the heating the rod and continuous stretching till intermediate substance of quartz tube is obtained with inner diameter of air core being as 1-3nm and outer diameter being as mm level; (2) using laser knife to cut intermediate substance fixed on the slicing machine along direction perpendicular to direction of longitudinal axis of the intermediate substance; thin film in 0.5-50 micros is cut out; (3) sticking the thin film to glass base plate to carry out grinding and Nano polishing till the thin film is ground to o.2 micro thickness; (4) taking off the thin film from the glass base plate, and fixing it to a solid phase holder, or fixing the thin film obtained from step (2) to the solid phase holder directly; sealing around the thin film to obtain the sensor needed. The invention solves issue of irregular Nano hole.

Description

technical field

[0001] The invention relates to a method in the technical field of nanomaterials, in particular to a method for manufacturing a biosensor. Background technique

[0002] There are natural protein holes in the protoplast membrane of organisms, which can perform intracellular and extracellular ion exchange. The ion flow can be detected by patch clamp, and the double lipid membrane is infected with α-hemolysin to form a bilateral asymmetry. The protein nanopores can also be used for the detection of ion currents. At present, they are widely used in the kinetic research of nucleic acid and single-stranded deoxynucleic acid (collectively referred to as nucleic acid) perforation, the detection of the size of nucleic acid molecular fragments, and the potential to determine their base sequences. However, since this kind of nanopore is a hole formed by protein on the double lipid membrane, it is easy to age, cannot withstand high voltage, permeability is greatly affect...