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Method for producing biological sensor

A technology of biosensor and manufacturing method, which is applied in the field of biosensor manufacturing, and can solve problems that cannot be directly used to detect ions or charged molecules with small diameters

Inactive Publication Date: 2012-07-18
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After literature search of the prior art, it was found that Sun, L. and Crooks, R.M. published the title in (Journal of the American Chemical Society ("Journal of the American Chemical Society", 2000, Volume 122, page 12340-12345) They reported their research results in the article "Single carbon nanotube membranes: A well-defined model for studying mass transport through nanoporous materials." Embed multi-walled carbon nanotubes in epoxy resin, and then cut into thin slices perpendicular to the direction of the nanotubes to obtain a film with a single hole diameter of 153nm and a thickness (hole depth) of 660nm, but the diameter of this nanocomponent is too large. It can only be used for the detection of polystyrene particles with a diameter of 100nm, but not directly for the detection of ions or charged molecules with smaller diameters such as nucleic acid perforation kinetics

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0014] Purchase a commercial hollow (1mm inner diameter) quartz glass rod, stretch it at 1200°C, stretch it 10 times thinner and cool it to room temperature, then heat the outer quartz glass rod to 1200°C and continue stretching, due to the hollowness of the quartz tube The initial inner diameter is known, so it is stretched repeatedly until the hollow inner diameter reaches 1nm, and the outer diameter is in the middle material of mm level or even cm level. Fix the intermediate material on a microtome, and cut a film with a thickness of 0.5 μm perpendicular to the longitudinal axis of the intermediate material with a laser knife. The films were mounted on glass substrates, ground and nanopolished until the films were ground to 0.2 μm. Then remove the film and fix it on a glass support with a small hole with a diameter of 2 μm in the middle, the nano hole should be in the middle of the small hole, and seal the edge of the film with the solid support to obtain a biosensor.

Embodiment 2

[0016] Purchase a commercial hollow (1mm inner diameter) quartz glass rod, stretch it at 1200°C, stretch it 10 times thinner and cool it to room temperature, then heat the outer quartz glass rod to 1200°C and continue stretching, due to the hollowness of the quartz tube The initial inner diameter is known, so it is stretched repeatedly until the hollow inner diameter reaches 2nm, and the outer diameter is in the middle material of mm or even cm level. Fix the intermediate material on a microtome, cut the film with a thickness of 50 μm perpendicular to the longitudinal axis of the intermediate material with a laser knife, and fix it on a bakelite support with a small hole in the middle with a diameter of 5 μm. The nanohole should be in the middle of the small hole. Seal the edge of the film with a solid support to obtain a biosensor.

Embodiment 3

[0018] Purchase a commercial hollow (inner diameter 1mm) quartz glass rod, stretch it at 1100°C, stretch it 10 times thinner and cool it to room temperature, then heat the outer quartz glass rod to 1100°C and continue stretching, due to the hollowness of the quartz tube The initial inner diameter is known, so it is stretched repeatedly until the hollow inner diameter reaches 3nm, and the outer diameter is in the middle material of mm or even cm level. Fix the intermediate material on a microtome, cut the film with a thickness of 25 μm perpendicular to the longitudinal axis of the intermediate material with a laser knife, and fix the film on a glass support with a small hole with a self-diameter of 10 μm in the middle, and the nanopore should be in the small hole In the middle, the edge of the film is sealed with a solid support, the biosensor.

[0019] The biosensors obtained in the above examples separate the electrophoresis tank and combine with the patch clamp, which can be...

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Abstract

The method includes steps: (1) stretching air core quartz glass rod, cooling the drawn down rod, the heating the rod and continuous stretching till intermediate substance of quartz tube is obtained with inner diameter of air core being as 1-3nm and outer diameter being as mm level; (2) using laser knife to cut intermediate substance fixed on the slicing machine along direction perpendicular to direction of longitudinal axis of the intermediate substance; thin film in 0.5-50 micros is cut out; (3) sticking the thin film to glass base plate to carry out grinding and Nano polishing till the thinfilm is ground to o.2 micro thickness; (4) taking off the thin film from the glass base plate, and fixing it to a solid phase holder, or fixing the thin film obtained from step (2) to the solid phaseholder directly; sealing around the thin film to obtain the sensor needed. The invention solves issue of irregular Nano hole.

Description

technical field [0001] The invention relates to a method in the technical field of nanomaterials, in particular to a method for manufacturing a biosensor. Background technique [0002] There are natural protein holes in the protoplast membrane of organisms, which can perform intracellular and extracellular ion exchange. The ion flow can be detected by patch clamp, and the double lipid membrane is infected with α-hemolysin to form a bilateral asymmetry. The protein nanopores can also be used for the detection of ion currents. At present, they are widely used in the kinetic research of nucleic acid and single-stranded deoxynucleic acid (collectively referred to as nucleic acid) perforation, the detection of the size of nucleic acid molecular fragments, and the potential to determine their base sequences. However, since this kind of nanopore is a hole formed by protein on the double lipid membrane, it is easy to age, cannot withstand high voltage, permeability is greatly affect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48C03B23/047
Inventor 王志民詹黎
Owner SHANGHAI JIAO TONG UNIV
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