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Liver function test

A technology of liver function and bile acid derivatives, which can be used in biological testing, testing pharmaceutical preparations, immunoassays, etc., and can solve problems such as expensive and complicated

Inactive Publication Date: 2007-07-04
NORGINE BV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is quite expensive and complicated to operate because it uses both specific antisera and specifically labeled conjugated bile acids for each conjugated bile acid measured in the sample

Method used

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Examples

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Embodiment Construction

[0053] CLF was synthesized according to the method described elsewhere [Mills et al.'s "Biochemistry and Biophysics Acta", (1991) 1115, 151], sterilized and tested for the presence of heat Original.

[0054] The study was carried out with 6 healthy volunteers (1 female, 5 males) aged between 30-53 years. Subjects were placed supine after an overnight fast. After the initial basal blood sample was taken, CLF was injected intravenously at a dose of 0.02 mg / kg body weight (b.w.) in saline over 15 seconds. At 2, 3, 4, 6, 10, 15, 20, 30, 60, and 90 minutes, venous blood samples were collected from an antecubital facing vein and placed in gel and lithium heparin containers (trade name Vacutainers, manufactured by Becton Dickinson Vacutainer Systems). The blood samples were then centrifuged and a 0.5 ml aliquot of plasma was removed and added to 3.5 ml methanol to precipitate plasma proteins, followed by centrifugation again. The supernatant (1 ml) was diluted to 3 ml with methan...

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Abstract

A method of determining the liver function of a patient, comprises the steps of (i) processing a series of blood samples which have passed through the liver of the patient and which have been collected at timed intervals after introducing an effective amount of a coloured or fluorescent bile acid derivative intravenously into the patient; and (ii) accessing the colour or fluorescence of bile acid derivative in each sample by: (a) processing the blood sample to obtain blood plasma containing the bile acid derivative, (b) measuring the colour or fluorescence of the bile acid derivative in the blood plasma, and (c) fitting the measurements obtained in step (b) to a plasma elimination curve and comparing the plasma elimination curve with plasma elimination curves obtained from individuals having known liver function, to arrive at a determination of liver function for the patient.

Description

[0001] This application is a divisional application. The filing date of the original application is August 3, 1998, the application number is 98810086.X (PCT / GB98 / 02322), and the title of the invention is "liver function test". technical field [0002] The present invention relates to methods of determining liver function (ie, enabling the identification of the nature of liver disease in test patients). Background technique [0003] The uptake and bile secretion of bile acids is an important hepatic function, and elevated serum bile acid concentrations are observed in several parenchymal and cholestatic liver diseases. Natural bile acids have been shown to correlate well between impaired plasma clearance and hepatic dysfunction in patients with various liver diseases [Korman et al. New Eng. J. Med.) (1975) 292, 1205] and rapid clearance from plasma of radiolabeled bile acids [Horak et al. Gastroenterology (1976) 71, 809]. P.-H.Ekdahl et al. ["atom light" (Atomlight) (1966) ...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/60G01N21/25G01N21/64A61K49/00A61B5/00G01N33/49C07D311/80C07J9/00G01N33/50G01N33/72
CPCA61K49/0052G01N33/728G01N33/583G01N33/582A61K49/0043Y10T436/146666G01N33/15
Inventor C·O·米尔斯
Owner NORGINE BV
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