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Human glutathione peroxidase-6

a technology of glutathione peroxidase and glutathione peroxidase, which is applied in the direction of biochemistry apparatus and processes, sugar derivatives, enzymes, etc., can solve the problems of clinical manifestations and imbalances, and achieve high concentration solutions, increase the solubility of compounds, and increase the viscosity of suspensions

Inactive Publication Date: 2002-03-14
INCYTE PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The use of GPx6 and its polynucleotides provides a means to address oxidative stress-related disorders by enhancing antioxidant defenses, allowing for effective diagnosis and treatment of reproductive, immune, and cell proliferative disorders.

Problems solved by technology

Disruption in this balance leads to clinical manifestations.

Method used

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  • Human glutathione peroxidase-6
  • Human glutathione peroxidase-6
  • Human glutathione peroxidase-6

Examples

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examples

[0180] I. PROSNOT20 cDNA Library Construction

[0181] The PROSNOT20 CDNA library was constructed using polyA RNA isolated from nontumorous prostate tissue removed from a 65-year-old Caucasian male during a radical prostatectomy. Pathology indicated adenofibromatous hyperplasia. Pathology for the associated tumor tissue indicated an adenocarcinoma (Gleason grade 2+2) involving the right anterior prostate peripherally. The patient presented with elevated prostate specific antigen (PSA).

[0182] The frozen tissue was homogenized and lysed in guanidinium isothiocyanate solution using a Brinkmann Polytron PT-3000 homogenizer (Brinkmann Instruments, Westbury N.Y.). The lysates were centrifuged over a 5.7 M CsCl cushion using a Beckman SW28 rotor in a Beckman L8-70M Ultracentrifuge (Beckman Instruments) for 18 hours at 25,000 rpm at ambient temperature. The RNA was extracted with acid phenol, precipitated using sodium acetate and ethanol, resuspended in RNAse-free water, and treated with DNase...

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Abstract

The invention provides a human glutathione peroxidase (GPx6) and polynucleotides which identify and encode GPx6. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of GPx6.

Description

[0001] This application is a DIVISIONAL application of U.S. application Ser. No. 09 / 088,549, filed Jun. 1, 1998, entitled HUMAN GLUTATHIONE PEROXIDASE-6, which is hereby expressly incorporated by reference.[0002] This invention relates to nucleic acid and amino acid sequences of a human glutathione peroxidase-6 and to the use of these sequences in the diagnosis, treatment, and prevention of reproductive, immune, and cell proliferative disorders.[0003] Eukaryotic cells extract energy and synthesize macromolecules by a complex series of oxidation-reduction reactions collectively referred to as aerobic metabolism. One consequence of aerobic metabolism is the production of free radicals in the form of superoxides (O.sub.2.sup..cndot.-) and hydroxyl ions (OH.cndot.). Superoxides are produced within cells by mitochondria and the endoplasmic reticulum as a consequence of "leakage" of electrons onto O.sub.2 from their correct paths in electron transfer chains. Hydroxyl ions are produced by ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/00A61K38/44C12N9/08
CPCC12N9/0065A61K38/00C12Y111/01009
Inventor HILLMAN, JENNIFER L.CORLEY, NEIL C.PATTERSON, CHANDRA
Owner INCYTE PHARMA INC