Suppression of non-biological motion
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[0069] The present invention pertains to a method for analyzing a cell by suppressing non-biological movement. The method comprises the steps of placing the cell in a solution having a viscosity enhancement medium. There is the step of measuring the motility of the cell. Multiple cells can be measured in parallel.
[0070] The viscosity enhancement medium can be methyl cellulose. The viscosity enhancement medium can be hyaluronic acid or chondroitin sulfate or cellulose ester or poly sacharide.
[0071] The placing step can include the step of placing the cell in the solution of between 0.1% to 0.2% by total volume of methyl cellulose for 2D analysis of motility. The methyl cellulose solution can have a concentration of between 0.1% and 1.2% methyl cellulose onto cells in culture medium to provide a layer of methyl cellulose-containing medium for 2D analysis of motility. The placing step can include the step of placing the cell in the solution having a viscosity of 100-5000 centipoise. ...
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