Nucleic acid and amino acid sequences relating to Bacteroides fragilis for diagnostics and therapeutics

Abstract

The invention provides isolated polypeptide and nucleic acid sequences derived from Bacteroides fragilis that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.

Description

RELATED APPLICATIONS [0001] This application is a divisional of U.S. application Ser. No. 09 / 540,209, filed Apr. 4, 2000 which claims the benefit of U.S. Provisional Application No. 60 / 128,705, filed Apr. 9, 1999, the entire teachings of which are incorporated herein by reference.FIELD OF THE INVENTION [0002] The invention relates to isolated nucleic acids and polypeptides derived from Bacteroides fragilis that are useful as molecular targets for diagnostics, prophylaxis and treatment of pathological conditions, as well as materials and methods for the diagnosis, prevention, and amelioration of pathological conditions resulting from bacterial infection. BRIEF DESCRIPTION OF TABLE 2 [0003] Incorporated herein by reference in its entirety is Table 2, comprising Line 1 to Line 10444. Table 2 contained on a CD-ROM, two copies of which are filed, the Table being in a computer-readable ASCII file “Table 2.txt” created on Feb. 24, 2005 and of 583 bytes in size, in IBM-PC Windows®NT v4.0 fo...

AI Technical Summary

Benefits of technology

[0016] A variety of data storage structures are available to a person skilled in the art for creating a computer readable media having recorded thereon a nucleotide sequence of the present invention. The choice of the data storage structure will generally be based on the means chosen to access the stored information. In addition, a variety of data processor programs and formats can be used to store the nucleotide sequence information of the present invention on computer readable media. The sequence information can be represented in a word processing text file, formatted in commercially-available software such as WordPerfect and Microsoft Word, or represented in the form of an ASCII file, stored in a database application, such as DB2, Sybase, Oracle, or the like. A person skilled in the art can readily adapt any number of data processor structuring formats (e.g. text file or database) in order to obtain computer readable media having recorded thereon the nucleotide sequence information of the present invention.

[0214] The nucleic acid sequence of the present invention may be used to detect B. fragilis or other species of Bacteroides acid sequence using bio chip technology. Bio chips containing arrays of nucleic acid sequence can also be used to measure expression of genes of B. fragilis or other species of Bacteroides. For example, to diagnose a patient with a B. fragilis or other Bacteroides infection, a sample from a human or animal can be used as a probe on a bio chip containing an array of nucleic acid sequence from the present invention. In addition, a sample from a disease state can be compared to a sample from a non-disease state which would help identify a gene that is up-regulated or expressed in the disease state. This would provide valuable insight as to the mechanism by which the disease manifests. Changes in gene expression can also be used to identify critical pathways involved in drug transport or metabolism, and may enable the identification of novel targets involved in virulence or host cell interactions involved in maintenance of an infection. Procedures using such techniques have been described by Brown et al., 1995, Science 270: 467-470.

Problems solved by technology

The increasing resistance to antibiotics commonly used against Bacteroides species may eventually lead to failures of these treatments.

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