Personal care composition containing ozone-stressed yeast lysates

Abstract

The present invention relates to an ozone-stressed yeast lysate, and a personal care composition including an ozone-stressed yeast lysate and at least one preservative selected from the group consisting of alcohols, glycols, parabens, hydantoins, quaternary nitrogen-containing compounds, isothiazolinones, aldehyde-releasing agents, and halogenated compounds. The present invention also relates to a method for preparing and using the ozone-stressed yeast lysate and the personal care composition.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims the benefit of U.S. Provisional Patent Application Ser. No. 60 / 618,698, filed on Oct. 13, 2004, entitled “Personal Care Composition Containing Ozone-Stressed Yeast Lysates”, the disclosure of which is incorporated herein by reference in its entirety.FIELD OF THE INVENTION [0002] This invention relates generally to personal care compositions, and more specifically to such compositions containing ozone-stressed yeast lysates. BACKGROUND OF THE INVENTION [0003] Ozone is increasingly becoming recognized as a detrimental agent for skin cells. Ozone can affect lipids, proteins, nucleic acids and sugars but is especially severe to key antioxidants located in skin cells. The damage appears to be most severe at the surface layers of skin, and diminishes as one probes into deeper dermal and epidermal layers. Ozone, which is generated by a number of natural sources such as lightning, is also generated by various industrial ...

AI Technical Summary

Benefits of technology

[0043] Two-dimensional electrophoresis was performed according to the method of O'Farrell (J. Biol. Chem. 250: 4007-4021, 1975) by Kendrick Labs, Inc. (Madison, Wis.) as follows: Isoelectric focusing was carried out in glass tubes of inner diameter 2.0 mm using pH 4-8 2.0% ampholines (Gallard Schlesinger Industries, Inc. Garden City, N.Y.) for 9600 volt-hrs. Fifty nanograms of an IEF internal standard, tropomycin, was added to each sample. Tropomycin shows two polypeptide spots of similar pI (ionic charge); the lower spot of MW 33,000 and pI 5.2 was marked with an arrow on the stained gels. The tube gel pH gradient plot for this set of ampholine was determined with a surface pH electrode.

[0044] After equilibration for 10 min in buffer “0” (10% glycerol, 50 mm dithiothreitol, 2.3% SDS and 0.062 M tris, pH 6.8), each tube gel was sealed to the top of a stacking gel that was on top of a 12% acrylamide slab gel (0.75 mm thick). SDS slab gel electrophoresis was carried out for 4 hours at 12.5 mA/gel. The following proteins from Sigma were added as molecular weight standards to the agarose that sealed the tube gel to the slab gel: myosin (220,000), phosphorylase A (94,000), catalase (60,000), actin (43,000), carbonic anhydrase (29,000) and lysozyme (14,000). These standards appear along the basic edge of the silver-stained 12% acrylamide slab gel.

[0045] Duplicate gels were obtained by the 2-D methodology as described above. One gel from each pair was scanned with a laser densitometer (Model

Problems solved by technology

Ozone can affect lipids, proteins, nucleic acids and sugars but is especially severe to key antioxidants located in skin cells.

Deterioration of the lipid bi-layer increases trans-epidermal water loss and leads to drying and cracking of the skin.

Damage to nucleic acids can lead to cellular mutations and apoptosis of important skin cells.

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