Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Personal care composition containing ozone-stressed yeast lysates

Inactive Publication Date: 2006-05-25
ARCH PERSONAL CARE PROD
View PDF12 Cites 26 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015] Yet another aspect of the present invention relates to a method for preparing a personal care composition including an ozone-stressed yeast lysate effective in reducing or minimizing ozone-induced skin degradation when applied to skin cells. The method includes the steps of: growing yeast in a fermentation broth; exposing the yeast to ozone by aerating the yeast for a time period of between about five minutes and about seventy-two hours with an aeration gas having an ozone concentration of from about 0.0001 millimole (mM) to about 1.0 millimole (mM) based on the total volume of the fermentation broth to produce ozone-stressed yeast; lysing the ozone-stressed yeast to produce an ozone-stressed yeast lysate; and incorporating the ozone-stressed yeast lysate into a personal care composition.

Problems solved by technology

Ozone can affect lipids, proteins, nucleic acids and sugars but is especially severe to key antioxidants located in skin cells.
Deterioration of the lipid bi-layer increases trans-epidermal water loss and leads to drying and cracking of the skin.
Damage to nucleic acids can lead to cellular mutations and apoptosis of important skin cells.
Likewise, ozone can deteriorate proteins and sugars that comprise the extracellular matrix and natural moisturizers in the skin.
High concentration of ozone is typically lethal to yeast.
Despite previous attempts to increase oxygen uptake in human skin cells, there is no solution that directly addresses the need of ozone protection for the skin.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Personal care composition containing ozone-stressed yeast lysates
  • Personal care composition containing ozone-stressed yeast lysates
  • Personal care composition containing ozone-stressed yeast lysates

Examples

Experimental program
Comparison scheme
Effect test

example 1

Example of Yeast Lysate from Grown Saccharomyces

Organism and Media

[0038] The organism used in this study was S. cerevisiae (Red Star baker's yeast). Stock culture was maintained on a yeast peptide dextrose (YPD) agar slant (Difco). The working culture was maintained in YPD broth at 4° C. The fermentation was carried out with the medium containing 10 g / L yeast extract, 8 g / L NH4SO4, 3 g / L KH2PO4, 2 g / L MgSO4, and 0.5 mL / L Antifoam A. Unless otherwise stated, the working volume for fermentation was 2 liters (L).

Bioreactor

[0039] A New Brunswick Bioflo 110 benchtop bioreactor (Edison, N.J.) equipped with automatic pH, temperature, agitation, dissolved oxygen (DO) and antifoam controls was used. The 2-L vessel was equipped with air in- and out-ports, alkali and medium addition ports, and effluent side ports. Medium pH was maintained at 5.5 by adding 4 M NaOH and / or 4 M H2SO4. Aeration was maintained at 1 vvm (volume of air / working volume of fermentor / min) and DO level was kept at 6...

example 2

Yeast Gene Microarray Analysis: Comparing Effects of Ozone Verses Hydrogen Peroxide on Gene Expression

[0063] To determine whether ozone caused yeast to respond differently than it responds to hydrogen peroxide, yeast was exposed to either ozone or hydrogen peroxide for the same length of time. The yeast was then subjected to microarray analysis to determine what genes were up-regulated and which were down-regulated as a result of the treatments. The following test protocols were employed in this study.

Yeast Cell Culture and Treatment

[0064] Cell cultures of S. cerevisiae were grown as described in more detail above. At the desired time after treatment, a 3-6 ml aliquot of the yeast culture was obtained and the cell density was measured spectrophotometrically at 600 nm. Culture samples having an Optical Density (OD) between 1 and 2 and were diluted with culture media as necessary to bring them within this range and the final volume of the aliquot was recorded. The aliquot was then...

example 3

In Vitro Protective Effect of Ozone-Stressed Yeast Lysate on Carmine Dye Oxidation

[0087] In-vitro studies were performed by the degradation of potassium indigotrisulfonic acid upon exposure to ozone (Wentworth Jr. et al. 2003). Samples of ozone-stressed (or ozone treated) yeast lysate, non-ozone-stressed treated yeast lysate, and distilled water were compared. Approximately 36 mL of indigo reagent was mixed with 100 mL of sample to give an absorbance of 0.5. The final concentrations of yeast lysate samples were around 2%. Samples were then purged with 1.5 L / min of ozone / air stream containing 10 mg / L of ozone for 10 minutes. Absorbance values for samples were measured at 600 nm at different time intervals. Ozone has the unique ability to cleave the double bond in indigo to give the colorless isatin sulfonic acid compound, see below.

[0088] Results from ozone exposure of water, non-ozone-stressed yeast lysate and ozone treated yeast lysate are shown in the photograph in FIG. 1. The ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Temperatureaaaaaaaaaa
Temperatureaaaaaaaaaa
Fractionaaaaaaaaaa
Login to View More

Abstract

The present invention relates to an ozone-stressed yeast lysate, and a personal care composition including an ozone-stressed yeast lysate and at least one preservative selected from the group consisting of alcohols, glycols, parabens, hydantoins, quaternary nitrogen-containing compounds, isothiazolinones, aldehyde-releasing agents, and halogenated compounds. The present invention also relates to a method for preparing and using the ozone-stressed yeast lysate and the personal care composition.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims the benefit of U.S. Provisional Patent Application Ser. No. 60 / 618,698, filed on Oct. 13, 2004, entitled “Personal Care Composition Containing Ozone-Stressed Yeast Lysates”, the disclosure of which is incorporated herein by reference in its entirety.FIELD OF THE INVENTION [0002] This invention relates generally to personal care compositions, and more specifically to such compositions containing ozone-stressed yeast lysates. BACKGROUND OF THE INVENTION [0003] Ozone is increasingly becoming recognized as a detrimental agent for skin cells. Ozone can affect lipids, proteins, nucleic acids and sugars but is especially severe to key antioxidants located in skin cells. The damage appears to be most severe at the surface layers of skin, and diminishes as one probes into deeper dermal and epidermal layers. Ozone, which is generated by a number of natural sources such as lightning, is also generated by various industrial ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14
CPCC12N1/063A61K8/9728A61Q19/08A61K8/14A61K8/553A61K8/73A61K2800/522A61K2800/524A61K2800/805A61Q19/00
Inventor GRUBER, JAMES VINCENT
Owner ARCH PERSONAL CARE PROD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com