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System, method, device, and computer program product for extraction, gathering, manipulation, and analysis of peak data from an automated sequencer

a technology of automated sequencers and computer programs, applied in the field of system, method, device and computer program products, can solve the problems of not having a tool available to allow the extensive and efficient retrieval of raw data, and achieve the effect of high throughput analysis of data

Inactive Publication Date: 2006-11-16
INST PASTEUR +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increasing number of samples analyzed, no tool is currently available to allow the extensive and efficient retrieval of this raw data.

Method used

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  • System, method, device, and computer program product for extraction, gathering, manipulation, and analysis of peak data from an automated sequencer
  • System, method, device, and computer program product for extraction, gathering, manipulation, and analysis of peak data from an automated sequencer
  • System, method, device, and computer program product for extraction, gathering, manipulation, and analysis of peak data from an automated sequencer

Examples

Experimental program
Comparison scheme
Effect test

example 1.1

Mice and Parasites

[0135] Eight-week old B10.D2 mice were purchased from Harlan UK Limited. The clone 1.49 L of Plasmodium berghei ANKA (Amani, V., M. I. Boubou, S. Pied, M. Marussig, D. Walliker, D. Mazier, and L. Renia. 1998. Cloned lines of Plasmodium berghei ANKA differ in their abilities to induce Experimental Cerebral Malaria. Infect. Immun 66:4093-4099.) was kindly given by Dr. Walliker (Institute of Genetics, Edinburg, UK) and is maintained in the laboratory on C57BL / 6J female mice. This clone induced in mice a neurological syndrome partly mimicking the one of human CM. Erythrocytic stages of the parasite were cryopreserved in liquid nitrogen as stabilates in Alserver's solution containing 10% glycerol. Infection was induced by intraperitoneal injection of 106 parasitized red blood cells. Between day 7 to day 10 after infection, 90% of the mice developed cerebral malaria characterized by ataxia, paralysis, deviation of the head and convulsions followed by deep coma and death...

example 1.2

Cell Preparation

[0136] Blood was obtained on heparin by retroorbital punction. Mononuclear cells were isolated on ficoll-Hypaque gradient (Pharmacia, France). Spleen was removed and cells suspended in 3% FCS-PBS. Red blood cells were lysed with ammonium chloride buffer (ACK) for five minutes at room temperature. Cell preparations were then washed twice with PBS. Lymphoid cells were counted using Malassez cell in presence of eosin to exclude dead cells.

example 1.3

TCRB Reprtoire

[0137] Total RNA was extracted from more than 90,000 mononuclear cells for each sample using the TRI REAGENT kit (Molecular Research Center, Cincinnati, Ohio). 20 μg of glycogen (Roche, Meylan, France) was used to ensure optimal precipitation of RNA and pellet visualization. Protocols for TCR BV-BC and BV-BJ CDR3 spectratyping have been described previously, which were utilized in the present example (Pannetier, C., M. Cochet, S. Darche, A. Casrouge, M. Zöller, and P. Kourilsky. 1993. The sizes of the CDR3 hypervariable regions of the murine T-cell receptor b chains vary as a function of the recombined germ-line segments. Proc. Natl. Acad. Sci. USA 90:4319-4323.; Pannetier, C., J. Even, and P. Kourilsky. 1995. The Immunoscope technique for analysis of TCR repertoire. In The human antigen T cell receptor: Selected protocols and applications. J. R. Oksenberg, Austin, Tex. 287-325.). BC, BV and BJ primer sequences were those described previously (Pannetier, C., M. Cochet...

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Abstract

The present invention relates to a novel computer program product to extract and gather peak information from an automated sequencer or bioinformatics tool into a peak database, and to manipulate and analyze the peak information within the database.

Description

REFERENCE TO COMPUTER PROGRAM LISTING APPENDIX [0001] Filed herewith in triplicate (labeled Copy 1.1, Copy 1.2, and Copy 1.3, respectively) is a computer program listing appendix on a compact disc read only memory (CD-ROM). The entire contents of the computer program listing appendix is incorporated herein by reference. [0002] Each of the three copies of the computer program listing appendix were created on Jul. 1, 2003, and each includes the following files: NameSizeTypeModifiedAdmin49KBTextJul. 1, 2002CAppFile.cp7KBCP fileFeb. 5, 2002CAppFile.h3KBH fileMar. 30, 2001CAppleScript.cp8KBCP fileJan. 29, 2001CAppleScript.h2KBH fileJan. 29, 2001CGel.cp6KBCP fileAug. 16, 2001CGel.h4KBH fileJan. 29, 2001CISEApeaksApp.cp19KBCP fileFeb. 7, 2002CISEApeaksApp.h2KBH fileJan. 29, 2001CISEApeaksApp.ppob0KBPPOB fileFeb. 4, 2002CISEApeaksApp.rsrc0KBRSRC fileJun. 19, 2001CISEApp.cp11KBCP fileApr. 20, 2001CISEApp.h2KBH fileMar. 27, 2001Common.ppob0KBPPOB fileNov. 10, 2000Constants.h5KBH fileJun. 20,...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G06F19/00G01N33/48G16B50/00C12N15/09C12Q1/02C12Q1/68G16B30/00G16B30/20
CPCG06F19/28G06F19/22G16B30/00G16B50/00G16B30/20
Inventor COLLETTE, ALEXISSIX, ADRIENPIED, SYLVIANECAZEAVE, PIERRE-ANDRE
Owner INST PASTEUR
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