Supercritical fluids processing: preparation of protein microparticles and their stabilisation

a technology of supercritical fluids and protein, applied in the direction of peptide/protein ingredients, bulk chemical production, peptide sources, etc., can solve the problems of low efficiency, thermal degradation, and inability to develop liquid formulations, and achieve the effect of enhancing the solubility of the “solvent”

Inactive Publication Date: 2007-12-20
NOAKES SUZANNE MARIE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0024] The term “modifier” is a substance, preferably a solvent, which enhances the solubility of the “solvent” in the supercritical fluid.

Problems solved by technology

. . ) degradation, very often the development of liquid formulations is precluded.
Concerning spray drying, thermal degradation, low efficiency, low yield and high levels of residual moisture are the main limitations of the technique.
Another problem is the difference in long term stability of analogous formulations obtained by different drying processes.
Although many sugars can prevent protein damage during dehydration, the products often have a short shelf life at room temperature due to the Maillard reaction.
Hence, referring to the above stabilisation hypothesis of dried proteins, it can be postulated that vitrification is one of the main issues for long term stability.
Although fine amorphous particles can be produced, this process requires warm air as a drying force that can lead to protein thermal degradation.
Moreover, low efficiency, low yield and high levels of residual moisture are other limitations.

Method used

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  • Supercritical fluids processing: preparation of protein microparticles and their stabilisation
  • Supercritical fluids processing: preparation of protein microparticles and their stabilisation
  • Supercritical fluids processing: preparation of protein microparticles and their stabilisation

Examples

Experimental program
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Effect test

example 1

Preparation of Alkaline Phosphatase (ALP) / Trehalose Co-Precipitate Particles

[0070] In this example, the method of the invention is used to co-precipitate mixtures of alkaline phosphatase (ALP) and trehalose.

[0071] Solutions containing ALP (SIGMA Chemicals) at concentration 0.2% w / w and trehalose (SIGMA Chemicals) at concentration within the range 0-2% w / w in deionized water were used.

[0072] The ALP / Trehalose ratios of the obtained powders were as follow: 1:10, 1:2 and 1:0. Carbon dioxide as supercritical fluid and ethanol as modifier were used.

[0073] The solution was fed into the particle formation vessel 22 by means of pump 10 at a flow rate of 0.2 g / min. Supercritical carbon dioxide was fed by means of pump 8 at a flow rate of 30 g / min, ethanol was fed by means of pump 9 to line 34 at a flow rate of 4 g / min and it was mixed with supercritical carbon dioxide prior to entry into the particle formation vessel.

[0074] The supercritical fluid was injected into the particle formatio...

example 2

Preparation of Lysozyme / Trehalose Co-Precipitate Particles

[0086] In this example, the method of the invention is used to prepare co-precipitate powders using lysozyme and trehalose.

[0087] Solutions containing lysozyme (SIGMA Chemicals) at concentration within 0.2-1% w / w and trehalose (SIGMA Chemicals) at concentration within the range 0-2% w / w in deionized water were used. The lysozyme / trehalose ratios of the obtained powders were as follows: 1:10, 1:5, 1:2, 1:1, 2:1, 4:1 and 1:0 (Table 2).

[0088] Carbon dioxide was used as supercritical fluid and ethanol as modifier.

[0089] The aqueous solution containing the enzyme and the stabiliser was fed into the particle formation vessel 22 by means of pump 10 at a flow rate of 0.2 g / min. Supercritical carbon dioxide was fed by means of pump 8 at a flow rate of 30 g / min, ethanol was fed by means of pump 9 to line 34 at a flow rate of 4 g / min and it was mixed with supercritical carbon dioxide before entering into the particle formation vesse...

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Abstract

A coprecipitate of protein or polypeptide and stabiliser obtained by a process for the co-precipitation of a substance with a stabiliser therefor, by a gas anti solvent process comprising introducing into a particle formation vessel a supercritical fluid pure or mixed with a modifier; and a solution comprising said substance and said stabiliser dissolved in a solvent; so as said solvent is extracted from the solution by said supercritical fluid and co-precipitation of the substance and stabiliser occurs. The process may be carried out using an apparatus comprising a particle formation vessel and a nozzle having a central orifice serving to introduce a solution of the substance and a plurality of outer orifices serving to carry a flow of supercritical fluid into the particle formation vessel, such that the solvent is extracted from the solution by the supercritical fluid and precipitation of micron sized particles of the substance / stabiliser occurs.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation of, and claims the benefit of, U.S. application Ser. No. 10 / 493,256, filed Sep. 13, 2004; which is a national stage application of PCT / EP02 / 11761, filed Oct. 21, 2002; which claims priority to European Application No. 01125048.7, filed Oct. 22, 2001; all of which are hereby incorporated herein by reference in their entireties.FIELD OF THE INVENTION [0002] The present invention relates to a method for protein and polypeptide precipitation by supercritical fluid processing and their protection and stabilisation against denaturation. BACKGROUND OF THE INVENTION [0003] The need for stable proteins and polypeptides for many applications is continuously increasing. This is particularly pronounced for therapeutic proteins in the pharmaceutical field. For the ease of both manufacturers and final users aqueous protein solutions are often the preferred form of administration. Moreover this is their common natura...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): C07K14/00C07K2/00C07K1/30C12N9/96C07K1/32
CPCC07K1/30Y02P20/54C07K1/02
InventorGENTILE, MARCOPALMA, CESARE DICESTA, MARIA CANDIDA
OwnerNOAKES SUZANNE MARIE