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Methods and kits for bioremediation of contaminated soil

a technology for bioremediation and soil, applied in biological water/sewage treatment, sustainable waste treatment, instruments, etc., can solve the problems of high loss, process kills in excess of 90% of the microorganisms, and non-target microorganisms can become a significant part of the final product, etc., and achieve the effect of reducing the time required

Inactive Publication Date: 2008-09-04
ADVANCED BIOLOGICAL SERVICES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention is about a system for treating wastewater by using microbes to remove pollutants. The invention includes a fermentation system with a fermentation tank that has aeration, mixing, and maintained within a given temperature range. The system can grow microbes on-site at a wastewater treatment plant, reducing shipping costs. The invention also includes a method for economically inoculating a wastewater treatment facility by depositing an inoculum of microbes and monitoring and controlling various parameters of the fermentation system. The invention can be used to remove contaminants from aqueous liquid and wastewater. The invention also provides a method for reducing the time required for the treatment process."

Problems solved by technology

These microbial products target various problems associated with the operation of the treatment systems.
These unwanted or non-target microbes can become a significant part of the final product.
Even with the use of cryo-protectants in the reconstituted mixture to protect the microbes, this process generally kills in excess of 90% of the microbes.
Air-drying, a post liquid fermentation process, is also used by some companies to stabilize the microbes, but still results in high losses and poor recovery of viable microbes.
Using current technologies, the application of exogenous microbes often has no effect or insufficient impact, resulting in the plant violating National Pollutant Discharge Elimination System (NPDES) permits issued by the Environmental Protection Agency (EPA), or violating other environmental regulations, including state or local environmental regulations, and environmental regulations of countries other than the United States.
Since the NPDES Permits are monthly, this only leaves about two weeks or less to identify that the NPDES Permit is jeopardized, which is insufficient time for the plant to address the problem.

Method used

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  • Methods and kits for bioremediation of contaminated soil
  • Methods and kits for bioremediation of contaminated soil
  • Methods and kits for bioremediation of contaminated soil

Examples

Experimental program
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Effect test

example 1

Large Scale System in General

[0087]One aspect of the current invention is the inoculation at the application point with about 10-100 times more of the target microbes than conventional technology. With this in mind, the dosage at the point of application is optimally at least about 104 cfu / mL. Based on this goal, the working volume of the main fermentation tank is optimally about 1 / 500 to 1 / 50,000 of the flow to be treated, while the post fermentation tank is about 2-100 times larger in working volume, and the pre-fermentation tank is about 50-500 lesser in working volume. The microbial inoculum can be about 1 / 100 to 1 / 1000 of the first tank used whether that is the pre-fermentation tank or the main fermentation tank. For example, for a 30 day supply of inoculum for a 40 MGD plant with a pre-fermentation tank, the inoculum required to be stored would be 1.2 gallons, or 5 gallons would last for about 3 months without replacement.

[0088]Table 4 shows the relationship between the quanti...

example 2

System for Single Pass Lagoon System

[0103]In a single pass lagoon system, the influent is first directed to a primary clarifier in which solids are allowed to settle. Then the wastewater is passed through an aerated lagoon, and then into a settling pond, before discharge. In the single pass system, there is a continuous flow of wastewater, and therefore, continuous treatment is desired so that each part of the waste steam is treated.

[0104]In a plant with a 14 MGD flow, a single pass lagoon system is inoculated from a 1000 gallon tank containing a concentration of 1.4×109 cfu / ml microorganisms. The initial concentration of microorganisms in the inoculated 4 million gallon aeration tank is 105 cfu / ml. Typically 105 to 107 cfu / ml microorganisms can be recovered from a single pass lagoon. The inoculum is therefore sufficient to obtain almost immediate treatment. If the lagoon has a 3 day design residence time and the inoculum is added to the waste stream at the front end, then the effec...

example 3

System for Activated Sludge System

[0109]In an activated sludge system, the influent is delivered to a primary clarifier in which solids are allowed to settle. The wastewater then passes to an aerated basin, and then to a secondary clarifier where sludge is recycled to pass through the aerated basin again. Due to the recycling in the activated sludge system, a holding tank is not necessary, although it may be desired as a back up.

[0110]In a typical activated sludge system, it may take 14 to 30 days to see an effect of bioaugmentation due to the low rates of inoculation of microbes. Using the multistage fermentation system of the present invention, an effect is seen in approximately 5 days. In a small plant (smaller wastewater flow) with a large fermentation tank, allowing a larger inoculum, an effect is seen in as little as 24 to 48 hours.

[0111]For example, a plant with an activated sludge system to treat its wastewater is treated using the fermentation system of the present inventio...

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Abstract

The present invention relates to apparatus, methods, and applications for treating wastewater, and more particularly to biological processes for removing pollutants from wastewater. This invention further relates to apparatus and methods for growing microbes on-site at a wastewater treatment facility, and for economically inoculating sufficient microbes to solve various treatment problems rapidly

Description

RELATED APPLICATIONS[0001]The present patent document is a continuation-in-part of application Ser. No. 09 / 737,718 filed Dec. 15, 2000, which claims the benefit of the filing date under 35 U.S.C. §119(e) of Provisional U.S. Patent Application Ser. No. 60 / 171,264 filed Dec. 16, 1999. All of the foregoing applications are hereby incorporated by reference.BACKGROUND[0002]In the treatment of wastewater, microorganisms mostly bacteria use the soluble organic matter in the water as a food source. The bacteria consume the organic compounds and convert them to carbon dioxide, water, and energy to produce new cells.[0003]The use of microbes for wastewater treatment and environmental clean up of contaminated soils is well known. Examples of this can be seen in industry, such as microbial products sold to biological wastewater treatment plants (WWTP) by United States based companies such as Novozymes of Salem, Va. (Novozymes Biologicals, Inc.); InterBio, Inc. of The Woodlands, Tex.; Sybron Cor...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C02F3/34C02F3/02C02F3/00C02F3/12C02F3/28
CPCC02F3/00G06Q10/30C02F3/12C02F3/28C02F3/34C02F3/348C02F2209/02C02F2209/05C02F2209/06C02F2209/08C02F2209/10C02F2209/11C02F2209/22C02F2209/42C02F2303/12C02F2305/06C02F3/006Y02W90/00Y02W10/10C02F3/30
Inventor WHITEMAN, G. ROBERT
Owner ADVANCED BIOLOGICAL SERVICES INC
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