Plant Defense Signal Peptides

a plant defense and signal peptide technology, applied in the field of plant defense signal peptides, can solve the problems of many interactions that are harmful to plants, damage to plants, and decimation of ornamental plants, etc., and achieve the effects of enhancing resistance to disease infestation, reducing disease symptoms, and improving yield of plant products

Inactive Publication Date: 2009-05-07
WASHINGTON STATE UNIV RES FOUND INC
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  • Abstract
  • Description
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Benefits of technology

[0022]We have identified a novel defense signal peptide from Arabidopsis and a variety of other plants, including many crop plants of commercial importance. In addition, we have identified genes encoding polypeptides that are processed in plant cells to produce the shorter peptides, as well as receptors for the peptides. Transgenic plants in which these defense signal peptides are expressed under the control of a heterologous promoter, such as a constitutive promoter, exhibit improved yield of plant product, reduced disease symptoms, and/or enhanced resistance to disease in

Problems solved by technology

Although many of the interactions between these organisms and plants, particularly via the roots of the plants, are beneficial, many of the interactions are harmful to the plants.
The decimation of agricultural crops, ornamental plants, and other plants by diseases caused by plant pathogens is a worldwide problem that has enormous economic impact.
Damage to plants is caused by pathogens of multiple genera.
The activity of these com

Method used

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  • Plant Defense Signal Peptides
  • Plant Defense Signal Peptides
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Examples

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example 1

Isolation and Analysis of AtPep1 and Paralogs and Orthologs thereof

[0186]Innate immunity is initiated in animals and plants through the recognition of a variety of pathogen associated molecules that in animals are called “pathogen-associated molecular patterns,” or PAMPS, and in plants are called elicitors. Peptides derived from pathogens can be powerful elicitors of plant defense responses (Hahlbrock et al., Proc. Natl. Acad. Sci. USA 92:4150-4157, 1995; van den Askerveken et al., Plant Physiol. 103:91-96, 1993; Kammpren, Curr. Opin. Plant Biol. 4:295-300, 2001; Kunze et al., Plant Cell, 16:3496-3507, 2004; Navarro et al., Plant Physiol. 135:1113-1128, 2004; Fellbrich et al., Plant J. 32:375-390, 2002; He et al., Cell 73:1255-1266, 1993), but plant-derived peptides have not been identified previously that are elicitors of immune responses directed against pathogens.

[0187]We have isolated and characterized a 23 amino acid peptide, called AtPep1, that is a signaling component of the ...

example 2

An LRR Receptor Kinase is a Component of AtPep1 Amplification of Innate Immunity in Arabidopsis

[0223]Over 200 LRR receptor kinases are present in the Arabidopsis genome. Only a few LRR receptor kinases have been identified that interact with the peptide signals in plants. This includes the receptor for the defense peptide signal, systemin (Scheer and Ryan, Proc. Natl. Acad. Sci. 99:9585-9590, 2002) and receptors for the developmental peptide signals CLAVATA1 (Clark and Meyerowitz, Cell 89:575-585, 1997), phytosulfokine-alpha (Matsubayashi et al., Science 296:1470-1472, 2002) and the pathogen-derived peptide flg22 (Gomez-Gomez and Boller, Trends Plant Sci. 7:251-256, 2000).

[0224]The discovery that the AtPep family of endogenous peptides from Arabidopsis leaf extracts cause an alkalinization of the medium of Arabidopsis suspension cultured cells indicates that the peptide plays a role in plant cells by interacting with a cell surface receptor. Investigations of the biological role of...

example 3

Shorter Peptides from the C-Terminus of AtPep1 Possess Substantial Defense Signal Peptide Activity

[0240]Analogs of AtPep1 from the C-terminus of AtPep1 were synthesized and assayed in the alkalinization assay. One mL aliquots of 4 day old Arabidopsis cells were allowed to equilibrate on an orbital shaker at 180 rpm for one hour. A ten μL aliquot of each peptide solution was added to the cells. Peptide concentrations of 0.25 nM, 2.5 mM, and 25 nM were tested. After 20 min, the pH of the media was recorded. The results are shown in FIG. 5. An analog of AtPep1 missing the carboxy-terminal amino acid was completely inactive, whereas deletions from the amino-terminus of the peptide resulted in a sequential reduction in activity, until peptides with 9 carboxy-terminal amino acids remaining (SSGRPGQHN) were inactive. A peptide with 10 carboxy-terminal amino acids remaining had substantial defense signal peptide activity at the 25 nM peptide concentration, causing a change in pH of over 0.2...

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Abstract

A 23 amino acid peptide, AtPtpl, plays an important role as a signaling component of the innate immune system of Arabidopsis. The peptide precursor gene is transcribed in response to elicitors generated by pathogens, and AtPep1 is produced to amplify the signaling pathways. Seven paralogs of the AtproPep1 gene have been identified in the Arabidopsis genome, and orthologs have been identified in species of several agriculturally important families. AtPcpl and its paralogs and orthologs play important roles as endogenous signals to amplify innate immunity. The sequence of two AtPep1 receptors from Arabidopsis are also provided.

Description

CROSS-REFERENCE TO RELATED CASES[0001]This application claims priority from U.S. provisional patent application Ser. No. 06 / 647,708, filed Jan. 26, 2005, which is incorporated herein by reference.STATEMENT OF GOVERNMENT RIGHTS[0002]The invention was supported, at least in part, by a grant from the Government of the United States of America (grant no. IBN 0090766 from the National Science Foundation). The Government may have certain rights to the invention.BACKGROUND[0003]1. Technical Field[0004]The present invention relates to materials and methods for enhancing plant disease resistance.[0005]2. Background Information[0006]Plants are exposed to numerous denizens of their environment, including bacteria, viruses, fungi, and nematodes. Although many of the interactions between these organisms and plants, particularly via the roots of the plants, are beneficial, many of the interactions are harmful to the plants. The decimation of agricultural crops, ornamental plants, and other plants...

Claims

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Application Information

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IPC IPC(8): A01H1/00C07K14/00C07K7/08C07K7/06A61K38/16A61K38/08C12Q1/68G01N33/68C40B30/00A61K38/10A01H5/00C07H21/00C12N5/10A01N57/16
CPCC07K14/415C12N15/8282C12N15/8279
Inventor RYAN, CLARENCE A.RYAN, PATRICIA LOUISEPEARCE, GREGORY L.HUFFAKER, ALISAYAMAGUCHI, YUBE
Owner WASHINGTON STATE UNIV RES FOUND INC
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