Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

M-csf specific monoclonal antibody and uses thereof

a monoclonal antibody and specific technology, applied in the field of mcsf specific monoclonal antibody, can solve the problems of high susceptibility, serious morbidity, and substantial refractory to cancer metastasis therapy, and achieve the effect of preventing or reducing bone loss

Inactive Publication Date: 2009-12-31
XOMA TECH LTD +1
View PDF17 Cites 36 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention provides non-murine monoclonal antibodies that specifically bind to the same epitope as murine antibodies RX1, MC1, and MC3. These antibodies can be used for various applications such as research, diagnosis, and treatment of diseases. The non-murine antibodies can be generated by immortalizing the monoclonal antibodies or by using CDRs of these antibodies. The invention also provides variants of the non-murine antibodies that have different amino acid sequences but still retain the same binding affinity. These antibodies can be used in combination with other molecules or techniques to improve their function and effectiveness."

Problems solved by technology

Despite intensive efforts to develop treatments, cancer metastasis remains substantially refractory to therapy.
The occurrence of osteolytic bone metastases causes serious morbidity due to intractable pain, high susceptibility to fracture, nerve compression and hypercalcemia.
Despite the importance of these clinical problems, there are few available treatments for bone loss associated with cancer metastasis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • M-csf specific monoclonal antibody and uses thereof
  • M-csf specific monoclonal antibody and uses thereof
  • M-csf specific monoclonal antibody and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0422]This example shows that M-CSF antibodies RX1 and 5A1 are species specific and that antibodies RX1, MC1, and MC3 neutralize human M-CSF activity. RX1 is a commercially sold antibody that was available more than a year prior to the filing date of this application. Exemplary commercial sources include, but are not limited to, mouse anti-human M-CSF monoclonal antibody clones 116, 692, and 21 (Anogen); anti-human M-CSF antibody clones 21113.131, 26730, and 26786 (R & D Systems, Inc.); and anti-human M-CSF antibodyclone M16 (Antigenix America, Inc.).

[0423]To test the neutralizing activity of RX1 and 5A1, a proliferation assay of M-NFS-60 cell line was used (American Type Culture Collection Accession No. CRL-1838, available from ATCC in Rockville, Md., USA, derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirous, responsive to both interleukin 3 and M-CSF and which contain a truncated c-myb proto-oncogene caused by the integration of a retro...

example 2

[0425]This example shows that antibody RX1 effectively inhibits osteolysis in a human xenograft model at a dose of 5 mg / kg. Female nude mice at the age of 4-7 weeks old, average weight ˜20 g were used in this study. Tumor cells (MDA-MB-23 1, 3×105) suspended in 10 μl of saline was be injected into the right tibia bone marrow cavity. Radiograms of the hind legs were taken one day after tumor inoculation for getting baseline image and checking for bone fracture caused by injection. Mice were randomized into treatment groups at 10 mice per group including PBS and RX1 at 5 mg / kg, injected i.p. once a week for 6 weeks. At the end of study, radiograms of the hind legs were taken again and compared against baseline for bone damage. The degree of bone damage caused by tumor was defined as shown in FIG. 6. The group with RX1 5 mg / kg treatment showed statistically significant protection of the bone from tumor-cased damage.

example 3

[0426]This example shows that the number of metastases is reduced when antibody RX1 is administered to human breast cancer MDA-MB-231 bearing nude mice at a concentration of 5 mg / kg.

[0427]Female nude mice at the age of 4-7 weeks old, average weight ˜20 g were used for this study. Tumor cells (MDA-MB-231, 3×105) suspended in 10 μl of saline was injected into the right tibia bone marrow cavity. Radiograms of the hind legs were taken one day after tumor inoculation for getting baseline image and checking for bone fracture caused by injection. Mice were randomly grouped into the treatment groups including PBS and RX1 at mg / kg injected i.p. once a week for 6 weeks. At the end of study, lungs of each treatment group were collected and fixed in Bouin's solution for metastatic lung nodule counting.

[0428]As shown in FIG. 7, that the number of metastases is reduced when antibody RX1 is administered to human breast cancer MDA-MB-231 bearing nude mice at a dose of 5 mg / kg.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
dissociation equilibrium constantaaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

M-CSF-specific antibody RX1 is provided, along with pharmaceutical compositions containing antibody RX1, kits containing a pharmaceutical composition, and methods of preventing and treating bone loss in a subject afflicted with an osteolytic disease.

Description

[0001]This application claims priority of U.S. Provisional Application No. 60 / 535,181, filed Jan. 7, 2004, and U.S. Provisional Application No. 60 / 576,417 filed Jun. 2, 2004, each of which is incorporated by reference herein in its entirety.TECHNICAL FIELD[0002]This invention relates to methods for preventing and treating osteolysis, cancer metastasis and bone loss associated with cancer metastasis by administering an M-CSF-specific antibody to a subject.BACKGROUND OF THE INVENTION[0003]Cancer metastasis is the primary cause of post-operation or post-therapy recurrence in cancer patients. Despite intensive efforts to develop treatments, cancer metastasis remains substantially refractory to therapy. Bone is one of the most common sites of metastasis of various types of human cancers (e.g., breast, lung, prostate and thyroid cancers). The occurrence of osteolytic bone metastases causes serious morbidity due to intractable pain, high susceptibility to fracture, nerve compression and hy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/395C07K16/00A61P35/00A61P19/00C07K16/24
CPCA61K2039/505C07K16/243C07K2316/96C07K2317/21C07K2317/24A61K39/3955C07K2317/73C07K2317/92C07K2317/34A61K31/663C07K2317/55C07K2317/51C07K2317/515C07K2317/54C07K2317/56C07K2317/622C07K2317/76A61K31/675A61P1/02A61P1/16A61P13/12A61P19/00A61P19/02A61P19/08A61P19/10A61P29/00A61P3/00A61P3/02A61P3/14A61P35/00A61P35/02A61P35/04A61P43/00A61P5/00A61P5/14C07K16/00C07K16/18C07K16/24A61K39/39558A61K45/06C07K2317/565C07K2317/52
Inventor LIU, CHENGZIMMERMAN, DEBORAH LEEHARROWE, GREGORY M.KOTHS, KIRSTONKAVANAUGH, WILLIAM M.LONG, LIHORWITZ, ARNOLDCALDERON-CACIA, MARIA
Owner XOMA TECH LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com