Loc device for electrochemiluminescent detection of target nucleic acid sequences using hybridization chamber array and negative control chamber without probes

a technology of electrochemiluminescent detection and locator, which is applied in the field of diagnostic devices, can solve the problems of slow growth of this type of testing in the clinical laboratory, reduced sensitivity, and high degree of non-specific binding, and achieves the effects of simplifying the detector circuitry, improving the reliability of analytical results, and compact and inexpensive assay systems

Inactive Publication Date: 2011-12-22
GENEASYS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0092]The hybridization array provides for analysis of the targets via hybridization, with the control probes improving the reliability of the analytical outcomes.
[0093]The electrochemiluminescence-based assay target detection obviates any need, of the assay system, for an excitation light source, excitation optics, and optical filter elements, in turn, providing for a more compact and more inexpensive assay system. The absence of the requirement for the rejection of any excitation light also simplifies the detector circuitry, making the assay system even more inexpensive.

Problems solved by technology

Insufficient stringency can result in a high degree of nonspecific binding.
Excessive stringency can lead to a failure of appropriate binding, which results in diminished sensitivity.
Despite the advantages that molecular diagnostic tests offer, the growth of this type of testing in the clinical laboratory has been slower than expected and remains a minor part of the practice of laboratory medicine.
This is primarily due to the complexity and costs associated with nucleic acid testing compared with tests based on methods not involving nucleic acids.
However, controlling fluid flow through the LOC device, adding reagents, controlling reaction conditions and so on necessitate bulky external plumbing and electronics.
Connecting a LOC device to these external devices effectively restricts the use of LOC devices for molecular diagnostics to the laboratory setting.
The cost of the external equipment and complexity of its operation precludes LOC-based molecular diagnostics as a practical option for point-of-care settings.

Method used

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  • Loc device for electrochemiluminescent detection of target nucleic acid sequences using hybridization chamber array and negative control chamber without probes
  • Loc device for electrochemiluminescent detection of target nucleic acid sequences using hybridization chamber array and negative control chamber without probes
  • Loc device for electrochemiluminescent detection of target nucleic acid sequences using hybridization chamber array and negative control chamber without probes

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Embodiment Construction

Overview

[0234]This overview identifies the main components of a molecular diagnostic system that incorporates embodiments of the present invention. Comprehensive details of the system architecture and operation are set out later in the specification.

[0235]Referring to FIGS. 1, 2, 3, 104 and 105, the system has the following top level components:

[0236]Test modules 10 and 11 are the size of a typical USB memory key and very cheap to produce. Test modules 10 and 11 each contain a microfluidic device, typically in the form of a lab-on-a-chip (LOC) device 30 preloaded with reagents and typically more than 1000 probes for the molecular diagnostic assay (see FIGS. 1 and 104). Test module 10 schematically shown in FIG. 1 uses a fluorescence-based detection technique to identify target molecules, while test module 11 in FIG. 104 uses an electrochemiluminescence-based detection technique. The LOC device 30 has an integrated photosensor 44 for fluorescence or electrochemiluminescence detection...

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Abstract

A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for detection of the targets, and a pair of the electrodes, and, at least one negative control chamber without the ECL probes.

Description

FIELD OF THE INVENTION[0001]The present invention relates to diagnostic devices that use microsystems technologies (MST). In particular, the invention relates to microfluidic and biochemical processing and analysis for molecular diagnostics.CO-PENDING APPLICATIONS[0002]The following applications have been filed by the Applicant which relate to the present application:GBS001USGBS002USGBS003USGBS005USGBS006USGSR001USGSR002USGAS001USGAS002USGAS003USGAS004USGAS006USGAS007USGAS008USGAS009USGAS010USGAS012USGAS013USGAS014USGAS015USGAS016USGAS017USGAS018USGAS019USGAS020USGAS021USGAS022USGAS023USGAS024USGAS025USGAS026USGAS027USGAS028USGAS030USGAS031USGAS032USGAS033USGAS034USGAS035USGAS036USGAS037USGAS038USGAS039USGAS040USGAS041USGAS042USGAS043USGAS044USGAS045USGAS046USGAS047USGAS048USGAS049USGAS050USGAS054USGAS055USGAS056USGAS057USGAS058USGAS059USGAS060USGAS061USGAS062USGAS063USGAS065USGAS066USGAS067USGAS068USGAS069USGAS070USGAS080USGAS081USGAS082USGAS083USGAS084USGAS085USGAS086USGAS087USGAS...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C40B40/06G01N21/76
CPCB01L3/5027Y10T436/25B01L3/502738B01L7/52B01L2200/10B01L2300/023B01L2300/024B01L2300/0636B01L2300/0654B01L2300/0883B01L2300/10B01L2300/1827B01L2400/0406B01L2400/0633B01L2400/0677B01L2400/0688F16K99/003F16K99/0036G01N27/223C12Q1/68Y10T436/107497Y10T436/173845Y10T436/143333Y10T436/11Y10T436/145555Y10T436/203332Y10T436/25375B01L3/502707Y10T137/0352Y10T137/0391Y10T137/1044Y10T137/206Y10T137/2076Y10T137/2202Y02A90/10
Inventor MOINI, ALIREZAAZIMI, MEHDISILVERBROOK, KIA
Owner GENEASYS
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