Microfluidic device for detection of mitochondrial DNA via electrochemiluminescence modulated hybridization

a microfluidic device and mitochondrial dna technology, applied in the field of diagnostic devices, can solve the problems of slow growth of this type of testing in the clinical laboratory, reduced sensitivity, and high degree of non-specific binding, and achieves the effects of improving the specificity of the detection of target molecules, compact and inexpensive assay systems, and high sensitivity and fidelity

Inactive Publication Date: 2011-12-22
GENEASYS
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Benefits of technology

[0090]Detection of mitochondrial DNA sequences has the advantage of maintaining high sensitivity and fidelity in spite of old or degraded sample material. This increases the specificity of the detection of target molecules. This enables more sensitive, and more specific, detection of target DNA. An advantage conferred by the signal change based on complementarity is the ability to obtain a signal in a homogeneous format. No washing, additional sensitisation, or development steps are req

Problems solved by technology

Insufficient stringency can result in a high degree of nonspecific binding.
Excessive stringency can lead to a failure of appropriate binding, which results in diminished sensitivity.
Despite the advantages that molecular diagnostic tests offer, the growth of this type of testing in the clinical laboratory has been slower than expected and remains a minor part of the practice of laboratory medicine.
This is primarily due to the complexity and costs associated with nucleic acid testing compared with tests based

Method used

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  • Microfluidic device for detection of mitochondrial DNA via electrochemiluminescence modulated hybridization
  • Microfluidic device for detection of mitochondrial DNA via electrochemiluminescence modulated hybridization
  • Microfluidic device for detection of mitochondrial DNA via electrochemiluminescence modulated hybridization

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Overview

[0233]This overview identifies the main components of a molecular diagnostic system that incorporates embodiments of the present invention. Comprehensive details of the system architecture and operation are set out later in the specification.

[0234]Referring to FIGS. 1, 2, 3, 105 and 106, the system has the following top level components:

[0235]Test modules 10 and 11 are the size of a typical USB memory key and very cheap to produce. Test modules 10 and 11 each contain a microfluidic device, typically in the form of a lab-on-a-chip (LOC) device 30 preloaded with reagents and typically more than 1000 probes for the molecular diagnostic assay (see FIGS. 1 and 105). Test module 10 schematically shown in FIG. 1 uses a fluorescence-based detection technique to identify target molecules, while test module 11 in FIG. 105 uses an electrochemiluminescence-based detection technique. The LOC device 30 has an integrated photosensor 44 for fluorescence or electrochemiluminescence detection...

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Abstract

A microfluidic device for analysis of mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with mitochondria containing mitochondrial DNA, a lysis section for lysing the mitochondria to release the mitochondrial DNA, electrochemiluminescent (ECL) resonance energy transfer probes for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, a photosensor for detecting the probe-target hybrids.

Description

FIELD OF THE INVENTION[0001]The present invention relates to diagnostic devices that use microsystems technologies (MST). In particular, the invention relates to microfluidic and biochemical processing and analysis for molecular diagnostics.CO-PENDING APPLICATIONS[0002]The following applications have been filed by the Applicant which relate to the present application:GBS001USGBS002USGBS003USGBS005USGBS006USGSR001USGSR002USGAS001USGAS002USGAS003USGAS004USGAS006USGAS007USGAS008USGAS009USGAS010USGAS012USGAS013USGAS014USGAS015USGAS016USGAS017USGAS018USGAS019USGAS020USGAS021USGAS022USGAS023USGAS024USGAS025USGAS026USGAS027USGAS028USGAS030USGAS031USGAS032USGAS033USGAS034USGAS035USGAS036USGAS037USGAS038USGAS039USGAS040USGAS041USGAS042USGAS043USGAS044USGAS045USGAS046USGAS047USGAS048USGAS049USGAS050USGAS054USGAS055USGAS056USGAS057USGAS058USGAS059USGAS060USGAS061USGAS062USGAS063USGAS065USGAS066USGAS067USGAS068USGAS069USGAS070USGAS080USGAS081USGAS082USGAS083USGAS084USGAS085USGAS086USGAS087USGAS...

Claims

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Application Information

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IPC IPC(8): C40B60/12C12M1/34
CPCB01L3/5027Y10T436/25B01L3/502738B01L7/52B01L2200/10B01L2300/023B01L2300/024B01L2300/0636B01L2300/0654B01L2300/0883B01L2300/10B01L2300/1827B01L2400/0406B01L2400/0633B01L2400/0677B01L2400/0688F16K99/003F16K99/0036G01N27/223C12Q1/68Y10T436/107497Y10T436/173845Y10T436/143333Y10T436/11Y10T436/145555Y10T436/203332Y10T436/25375B01L3/502707Y10T137/0352Y10T137/0391Y10T137/1044Y10T137/206Y10T137/2076Y10T137/2202Y02A90/10
Inventor FACER, GEOFFREY RICHARDMOINI, ALIREZASILVERBROOK, KIAAZIMI, MEHDI
Owner GENEASYS
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