A method and system for cryopreservation to achieve uniform viability and biological activity

a cryopreservation and biological activity technology, applied in the field of cryopreservation process, can solve the problems of cell damage during cryopreservation, significant loss of cell viability, and 80% or more loss of cell activity and viability
US20130111931A1Inactive Publication Date: 2013-05-09PRAXAIR TECH INC
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
PRAXAIR TECH INC
Publication Date
2013-05-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method and system for controlled rate freezing and nucleation of biological materials is provided. The presently disclosed system and method provides the ability to rapidly cool the materials contained in vials or other containers within a cooling unit via forced convective cooling and optionally simultaneous pressure drop using uniform and unidirectional flow of cryogen in proximity to the plurality of vials disposed within a cooling unit. The rapid cooling of the biological materials is achieved by precisely controlling and adjusting the temperature of the cryogen being introduced to the system as well as the chamber pressure as a function of time.
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Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] The present invention is a continuation-in-part application of U.S. patent application Ser. No. 12 / 266,760 filed Nov. 7, 2008 and also claims priority from U.S. provisional patent application Ser. No. 61 / 480,647 filed Apr. 29, 2011 the disclosure of both applications are also fully incorporated by reference herein.FIELD OF THE INVENTION

[0002] The present invention broadly relates to a cryopreservation process, and more particularly, to a method and system for providing controlled rate freezing and nucleation control of biological materials to minimize cell damage resulting from intercellular ice formation and solute effects that arise during the cryopreservation process.BACKGROUND

[0003] Cryopreservation is a process used to stabilize biological materials at very low temperatures. Previous attempts to freeze biological materials, such as living cells often results in a significant loss of cell viability and in some cases as much as 80% or mo...

Claims

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