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Reaction vessel for carrying out array processes

a technology for array processes and reaction vessels, which is applied in the direction of analytical material containers, laboratory glassware, instruments, etc., can solve the problems of high cost, high cost, and the need for special equipment for the device used, and achieve the effect of not being suitable for individual tests, high cost, and high cos

Inactive Publication Date: 2017-03-02
CLONDIAG GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

Enables efficient, cost-effective, and reproducible micro-array based detection tests using standard laboratory equipment, reducing contamination risks and technical complexity, while allowing for the use of low-cost detection methods.

Problems solved by technology

In order to ensuring the tempering and mixing of the hybridisation solution in these hitherto known chambers, equipment specially adapted for the device used, which is therefore expensive and costly, is required.
The provision of such a device is extremely expensive and costly.
The reaction vessel plate described there is not suitable for carrying out individual tests.
A disadvantage with the conventional detection methods, however, is the considerable technical effort in some cases and the high costs associated with the detection method.

Method used

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  • Reaction vessel for carrying out array processes
  • Reaction vessel for carrying out array processes
  • Reaction vessel for carrying out array processes

Examples

Experimental program
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Effect test

example 1

Manufacture of the Reaction Vessel (1)

[0240]A standard reaction test tube from Eppendorf made of polypropylene and having a nominal receiving volume of 1.5 ml was used for re-melting. For this purpose the reaction tube was capped on the bottom side and some material was turned off from the sides. The tube was then placed on a pin and pressed under force onto a hot mould which impressed the opening or the recess (8), the chip support (6) as well as the adhesive edge (7) into the tube (see FIG. 1).

example 2

Detection and Specificity of the Hybridisation of Nucleic Acids in the Reaction Vessel (1) Using Silver Detection

[0241]a) Test System

[0242]The human cyp2D6 gene codes for a human cytochrome P450. This enzyme plays an important role in the metabolism of various drugs and active agents. Mutations in the cyp2D6 gene may result in hypersensitivity or intolerance for certain medicaments. At least 14 of such mutations (point mutations and deletions) are described for cyp2D6. For a selection of these mutations (G1749C, dT1795, G1934A, G2064A) it is to be tested whether the respective wild type or the mutations are present in a sample to be analysed.

[0243]b) Production of the DNA Library (102) and Assembly with the Reaction Vessel (1)

[0244]Using a MicroGrid II Arrayer (BioRobotics, Cambridge, Great Britain), 40 amino-modified oligonucleotides (probes) having a length of 21-25 nucleotides were deposited at defined sites on an epoxidized glass surface (slide size: 75 mm×25 mm) (101) and coval...

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Abstract

The invention relates to a reaction vessel, a device and a method for detecting specific interactions between molecular target and probe molecules. The present invention especially relates to a reaction vessel which has a shape and size typical of a laboratory reaction vessel and in which a supporting element with probe molecules immobilised thereon on predetermined regions is arranged on its base surfaces.

Description

BACKGROUND OF THE INVENTION[0001]Biomedical tests are frequently based on detecting an interaction between a molecule, which is present in known quantity and position (i.e. the molecular probe) and an unknown molecule to be detected or unknown molecules to be detected (i.e. the molecular target molecules). In modern tests the probes are deposited in the form of a substance library on supports, the so-called micro-arrays or chips so that one sample can be analysed simultaneously in parallel on a plurality of probes (D. J. Lockhart, E. A. Winzeler, Genomics, gene expression and DNA arrays; Nature 2000, 405, 827-836). In order to fabricate the micro-arrays the probes are usually immobilised in a predetermined manner on a suitable matrix, described for example in WO 00 / 12575 (see for example U.S. Pat. No. 5,412,087, WO 98 / 36827) or are produced synthetically (see for example U.S. Pat. No. 5,143,854).[0002]The interaction between the probe and the target molecule is usually detected as f...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68B01J19/00B01L3/00B01L3/14B01L7/00C12M1/00C12N15/09C12Q1/6837C40B40/02C40B40/06C40B50/06C40B60/12C40B60/14C40B70/00G01N21/17G01N33/53G01N33/543G01N37/00
CPCC12Q1/6837B01J2219/00283B01J2219/00529B01J2219/0054B01J2219/00585B01J2219/00596B01J2219/00707B01J2219/00722B01L3/50B01L3/5082B01L7/52B01L2300/0636B01L2300/0851C40B40/06C40B60/14C40B70/00G01N33/54366G01N21/253C12Q2547/101
Inventor SCHULZ, TORSTENERMANTRAUT, EUGENEHRICHT, RALFMOBIUS, KLAUS- PETERWAGNER, GERDFISCHER, JOACHIMELLINGER, THOMAS
Owner CLONDIAG GMBH