30 results about "Sperm binding protein" patented technology

The invention belongs to the field of biotechnology and medicine science, and provides sperm binding protein HEL-127 specifically expressed in human epididymis and a nucleic acid sequence for coding the protein. The invention also discloses a preparation method and application of the protein HEL-127. The protein is associated with the mature and defense function of sperms. The HEL-127 protein can be used for preparing medicaments of resisting infections and tumors, provides a novel molecular target for developing novel contraceptive medicine, carrying out aid diagnosis and treating infertility, and is favorable for researches on male reproductive function.

The invention belongs to biotechnology and medical field and discloses a new human epididymis specific expression sperm binding protein HEL-172 and preparation and application thereof. The invention discloses an amino acid sequence of the HEL-172 protein and provides a recombination expression vector carrying a DNA sequence for encoding the protein. The invention further discloses a method for preparing the protein and detecting the biological function of the HEL-172 protein. The protein is positioned at the back region of the head of a mature sperm, has expression in epididymis and spermary, heart, liver, spleen, kidney and stomach. A band of about 34 KD is detected in the epididymis by using Western blot. The HEL-172 protein can be used as a target protein for the research and development of immunity acyeterion as well as clinical diagnosis and treatment of male infertility. The relevant gene or protein detecting method based on the protein HEL-172 can be widely applied to the reproductive medicine research field.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-28 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-28 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located in the back region of the head of a mature sperm, and an RT-PCR result indicates that the protein has low expression level in an epididymis and various tissues of a humna body. A band of 187 KD is detected in the body part of the epididymis by using Western blot. The HEL-28 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-28 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-6 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-6 protein and provides a recombination expression carrier carrying the DNA sequence for encoding the protein. The invention also discloses a preparation method of the protein and a method for detecting the biological function of the HEL-6 protein. The protein is highly expressed in the head and tail parts of the epididymis, is combined at the head and tail parts of the sperm head and has important function for sperm-egg identification, fertilization and movement. The HEL-6 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-6 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-72 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-72 protein and provides a recombination expression carrier carrying the DNA sequence for encoding the protein. The protein is located in the primary segment and tail segment at the tail part of a mature sperm. A RT-PCR result shows that the gene is lowly expressed in the epididymis, highly expressed in the tissues of testis, kidney and stomach, and is negative in the heart and liver. A band of about 30 KD is detected in the corpus epididymidis part with Western blot. The HEL-72 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-72 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-97 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-97 protein and provides a recombination expression carrier carrying the DNA sequence for encoding the protein. The protein is located in the middle rear and tail parts of the head of a mature sperm. A RT-PCR result shows that the gene is weakly or rarely expressed in the head and tail part of the epididymis and other tissues of testis, heart, liver, spleen, kidney and stomach. A band of about 28 KD is detected in the corpus epididymidis part with Western blot. The HEL-97 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-97 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-47 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-47 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located in the neck region of a mature sperm, and an RT-PCR result indicates that the protein mainly has expression in an epididymis, a testis, a liver and tissues. A band of 47 KD is detected in the epididymis by using Western blot. The HEL-47 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-47 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the field of biology technology and medicine, and discloses a new human epididymis expression sperm binding protein HEL-122 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-122 protein and provides a recombination expression vector carrying a DNA sequence for coding the protein. The invention also discloses the preparation of the protein and a method for detecting the biology function of the HEL-122 protein. The protein is specifically expressed in the epididymis, and the expression quantity is highest in the epididymis part, and the protein is bound to the equator of the sperm to play a significant role in sperm fertilization. The HEL-122 protein can be used as a targeted protein for researching immunity contraceptives, and clinically diagnosing and treating male infertility. A corresponding gene or protein detection method developed based on the HEL-122 protein can be widely applied to the field of reproductive medicine research.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-61 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-61 protein and provides a recombination expression vector carrying the DNA sequence for encoding the protein. The protein is located in the middle tail area of the head of a mature sperm. A RT-PCR result shows that the gene is mainly expressed in the tissues of epididymis, testis, liver and spleen, and is negative in the kidney and stomach expression. A band of about 21 KD is detected in the corpus epididymidis body part with Western blot. The HEL-61 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-61 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-207 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-207 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located at the back part of the head of a mature sperm, an RT-PCR result indicates that the gene has expression in an epididymis tissue, a testis tissue, a cardiac tissue, a lung tissue, a liver tissue and a spleen tissue, and a band of about 46 KD is detected in an epididymis by using Western blot. The HEL-207 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-207 protein can be widely used for the research field of reproductive medicine.

The invention belongs to biotechnology and medical field, and discloses a new human epididymis specfic expression sperm binding protein HEL-190 and preparation and application thereof. The invention discloses an amino acid sequence of HEL-190 protein and provides a recombination expression vector carrying a DNA sequence encoding the protein of the invention. After the protein is positioned at the head of a mature sperm, an RT-PCR shows that specific expressions are mainly in various tissues of epididymis, spermary, liver, spleen, kidney and stomach, but the expression in heart is negative. A band of about 21 KD is detected in the epididymis by Western blot. The HEL-190 protein can be used as a target protein for the research and development of immunity acyeterion as well as clinical diagnosis and treatment of male infertility. The relevant gene or protein detecting method based on the protein HEL-190 can be widely applied to the reproductive medicine research field.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-43 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-43 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located in the equatorial region of a mature sperm, and an RT-PCR result indicates that the gene has weak expression in an epididymis and has no expression in other tissues. A band of 50 KD is detected in the body part of the epididymis by using Western blot. The HEL-43 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-43 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-202 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-202 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located at the back part of the head of a mature sperm and mainly has trace expression in an epididymis tissue and a testis tissue indicated by RT-PCR. A band of about 25 KD is detected in the epididymis by using western blot. The HEL-202 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-202 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-5 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-5 protein and provides a recombination expression vector carrying the DNA sequence for encoding the protein. The invention also discloses a preparation method of the protein and a method for detecting the biological function of the HEL-5 protein. The protein has low-abundance expressed in the epididymis, is combined on the surface of sperm and has important function for protecting the sperm, normal fertilization and early embryonic development. The HEL-5 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-5 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-90 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-90 protein and provides a recombination expression carrier carrying the DNA sequence for encoding the protein. The protein is located in the middle rear and tail parts of a mature sperm. A RT-PCR result shows that the gene is mainly and specifically expressed in the epididymis and testis and is negative in the heart, liver, spleen, kidney and stomach. A band of about 24 KD is detected in the corpus epididymidis part with Western blot. The HEL-90 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-90 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-40 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-40 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located at the back region of the head of a mature sperm, and an RT-PCR result indicates that the gene has higher expression level at the body part of an epididymis, a testis and a liver and has no expression in other tissues. A band of about 37 KD is detected in the body part of the epididymis by using Western blot. The HEL-40 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-40 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-78 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-78 protein and provides a recombination expression carrier carrying the DNA sequence for encoding the protein. The protein is located in the middle top and tail parts of a mature sperm. A RT-PCR result shows that the gene is mainly and specifically expressed in the epididymis. A band of about 72 KD is detected in the corpus epididymidis part with Western blot. The HEL-78 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-78 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-81 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-81 protein and provides a recombination expression carrier carrying the DNA sequence for encoding the protein. The protein is located in the rear area of the head of a mature sperm. A RT-PCR result shows that the gene is weakly expressed in the epididymis and heart. A band of about 44 KD is detected in the corpus epididymidis part with Western blot. The HEL-81 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-81 protein can be widely used for the research field of reproductive medicine.

The invention belongs to biotechnology and medical field, and discloses a new human epididymis specific expression sperm binding protein HEL-18 and preparation and application thereof. The invention discloses an amino acid sequence of the HEL-18 protein and provides a recombination expression vector carrying a DNA sequence encoding the protein of the invention. The invention further discloses a method for preparing the protein and detecting the biological function of the HEL-6 protein. The protein has micro-expression in the epididymis, heart and lung tissue, and is combined with the back part of the head of the sperm, thereby playing an important role in fecundation, recognition and movement of the sperm. The HEL-18 protein can be used as target protein for the research and development of immunity acyeterion as well as clinical diagnosis and treatment of male infertility. The relevant gene or protein detecting method based on the protein HEL-18 can be widely applied to the reproductive medicine research field.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-65 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-65 protein and provides a recombination expression carrier carrying the DNA sequence for encoding the protein. The protein is located in the primary segment and tail segment at the tail part of a mature sperm. A RT-PCR result shows that the gene is mainly expressed in the tissues of epididymis, heart and kidney and is negative in the testis, liver, spleen and kidney. A band of about 34 KD is detected in the corpus epididymidis part with Western blot. The HEL-65 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-65 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-200 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-200 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The invention also discloses a preparation method of the protein and a method for detecting the biological functions of the HEL-200 protein. The protein is located at the back part of the head of a sperm and the tail middle section of the sperm and has low abundance expression in an epididymis, a testis and a liver, and a band of about 35 KD is detected in the epididymis by using western blot. The HEL-200 protein can be used as a target protein or developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-200 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-45 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-45 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located in the neck region of a mature sperm, and an RT-PCR result indicates that the protein mainly has the highest expression in an epididymis. A band of 47 KD is detected in the epididymis by using Western blot. The HEL-45 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-45 protein can be widely used for the research field of reproductive medicine.

The invention belongs to biotechnology and medical field, and discloses a new human epididymis specific expression sperm binding protein HEL-178 and preparation and application thereof. The invention discloses an amino acid sequence of the HEL-178 protein and provides a recombination expression vector carrying a DNA sequence encoding the protein. The protein is positioned at the back region of the head of a mature sperm, an RT-PCR shows that higher micro-expression is mainly in epididymis, spermary, liver and spleen. A band of about 49 KD is detected the epididymis by using Western blot. The HEL-178 protein can be used as a target protein for the research and development of immunity acyeterion as well as clinical diagnosis and treatment of male infertility. The relevant gene or protein detecting method based on the protein HEL-178 can be widely applied to the reproductive medicine research field.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-9 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-9 protein and provides a recombination expression carrier carrying the DNA sequence for encoding the protein. The protein is located in the rear part of the head of the mature sperm, is highly expressed in the epididymidis, testis, heart, liver, spleen and kidney and is rarely expressed in the stomach. A band of about 50 KD is detected in the corpus epididymidis part with Western blot. The HEL-9 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility . A method for detecting a corresponding gene or protein based on development of the HEL-9 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-214 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-214 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located in a back region of the head of a mature sperm, has higher expression level in an epididymis, a testis and a liver and has lower expression level or no expression in other tissues. A band of about 15 KD is detected in the epididymis by using Western blot. The HEL-214 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-214 protein can be widely used for the research field of reproductive medicine.

The invention belongs to biotechnology and medical field, and discloses a new human epididymis specific expression sperm binding protein HEL-192 and preparation and application thereof. The invention discloses an amino acid sequence encoding HEL-192 protein and provides a recombination expression vector carrying a DNA sequence encoding the protein. The invention further discloses a method for preparing the protein and detecting the biological function of the HEL-192 protein. The protein is positioned at the back region of the head and a neck region of a mature sperm, has expression in epididymis and liver, and a band of about 33 KD in the epididymis by using Western blot. The HEL-192 protein can be used as a target protein for the research and development of immunity acyeterion as well as clinical diagnosis and treatment of male infertility. The relevant gene or protein detecting method based on the protein HEL-192 can be widely applied to the reproductive medicine research field.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-38 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-38 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located at the main section region and the terminal segment region of the tail of a mature sperm, and the gene mainly has specific expression in an epididymis, indicated by RT-PCR. A band of about 35 KD is detected in the epididymis by using Western blot. The HEL-38 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-38 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-215 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-215 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located in the back part of the head of a mature sperm and the neck region of the mature sperm and has higher expression level at the head and the body part of an epididymis. A band of about 16 KD is detected in the epididymis by using Western blot. The HEL-215 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-215 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the field of biotechnology and medicine, and discloses a new human epididymis specific expression sperm binding protein HEL-89 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-89 protein and provides a recombination expression carrier carrying the DNA sequence for encoding the protein. The protein is located in the top area of a mature sperm. A RT-PCR result shows that the gene is highly expressed in the epididymis and testis. A band of about 20 KD is detected in the corpus epididymidis part with Western blot. The HEL-89 protein can be used as a target protein for researching and developing immunity contraceptive as well as clinical diagnosis and treatment for male sterility. A method for detecting a corresponding gene or protein based on development of the HEL-89 protein can be widely used for the research field of reproductive medicine.

The invention belongs to the biotechnology and the medical field and discloses a new human epididymal specific expression sperm binding protein HEL-44 as well as preparation and application thereof. The invention discloses an amino acid sequence of the HEL-44 protein and provides a recombinant expression vector which carries a DNA sequence used for encoding the protein. The protein is located in the equatorial region of a mature sperm, an RT-PCR result indicates that a gene has expression in various tissues such as an epididymis, a testis, a heart, a lung, a liver, a spleen, a kidney, a stomach and the like, and a band of 23 KD is detected in the body part of the epididymis by using Western blot. The HEL-44 protein can be used as a target protein for developing an immunological contraceptive and clinically diagnosing and treating male infertility. A method for detecting a corresponding gene or protein based on development of the HEL-44 protein can be widely used for the research field of reproductive medicine.