The invention discloses a preparation method of insecticidal and growth-promoting organic fertilizer special for blueberry. The preparation method includes: drying, smashing and sieving aloe, garlic, pepper, Rangoon creeper fruit, chrysanthemum flower and Cyrtomium rhizome to obtain a mixed material A, and performing supercritical CO2 treatment and concentration to obtain a solution B; mixing beta-cyclodextrin and distilled water well, and then performing microwave treatment to obtain a material C; pouring the solution B into the material C, stirring before standing, and fluidizing and coating to obtain a Chinese herbal medicine bacteriostatic agent; heating urea, adding potassium chloride, monopotassium phosphate, zinc sulfate, ferrous sulfate, borax and manganese sulfate, mixing, cooling, and smashing to obtain a material E; mixing straw residue, livestock and poultry excrement, mushroom residue, urea and microbial inoculant well, performing composting fermentation, drying, and smashing to obtain a material F; stirring the Chinese herbal medicine bacteriostatic agent, bentonite and water, adding polyvinyl alcohol, starch, the material E and the material F, mixing, granulating, drying, and cooling to obtain the insecticidal and growth-promoting organic fertilizer.