The invention relates to a method for solving camera intrinsic parameter by using the image of the center of a sphere and orthogonality. The method comprises: firstly, taking three pictures of a calibrated sphere from different directions and reading the images, extracting coordinates of three quadratic curves from the three images; according to imaging process of the sphere and the center of the sphere under the camera, solving to obtain image coordinates of three centers of spheres, and according to projections of the centers of spheres, calculating end points on orthogonal directions, and finally by using the end points on orthogonal directions, solving images of absolute conics, and according to the images of quadratic curves, and solving a constraint matrix of camera intrinsic parameters, so as to obtain the camera intrinsic parameter. By using the spheres which are targets, full-automatic calibration can be realized, and errors caused by measurement in a calibration process are reduced. Since the quadratic curve is a simpler and more global element, and projection contours of the spheres can be completely extracted from the images, calibration precision is improved in a camera calibration process.